DNASU Plasmid | C15orf38 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: C15orf38-AP3S2

Gene Name: C15orf38-AP3S2 readthrough

Original Clone ID:

38356

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288605

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 681nts

Open reading frame: 1 to 681

Reference Sequence Alignment

ATGAGCCGCATCTACCACGACGGCGCGCTCCGGAACAAGGCGGTGCAGAGCGTCCGGCTG
CCAGGGGCCTGGGACCCCGCCGCCCACCAGGGGGGAAATGGTGTCCTGCTGGAGGGAGAA
CTGATCGATGTATCTCGGCACAGCATCTTGGACACTCATGGCAGGAAGGAGCGCTACTAC
GTGCTGTATATCCGGCCCAGTCACATCCATCGCCGTAAATTCGACGCCAAGGGAAATGAA
ATCGAGCCCAACTTCAGCGCCACCAGGAAGGTGAACACGGGCTTCCTCATGTCGTCCTAC
AAGGTGGAAGCCAAGGGGGACACTGACAGGCTCACGCCCGAGGCGCTGAAGGGGCTGGTC
AACAAGCCAGAGCTGCTCGCGCTGACAGAGAGCCTCACCCCCGACCACACAGTGGCGTTC
TGGATGCCCGAGTCAGAGATGGAGGTGATGGAACTCGAGCTGGGGGCCGGGGTACGGCTG
AAGACTCGGGGCGATGGTCCCTTCCTGGATTCATTGGCCAAACTTGAGGCTGGAACAGTG
ACCAAGTGTAATTTCACTGGTGATGGAAAGACAGGGGCATCCTGGACAGACAACATCATG
GCCCAAAAGTGTTCGAAGGGGGCTGCAGCGGAGATCCGAGAGCAGGGGGATGGGGCAGAG
GACGAGGAGTGGGATGACTGC

Protein sequence predicted based on nucleotide sequence

MSRIYHDGALRNKAVQSVRLPGAWDPAAHQGGNGVLLEGELIDVSRHSILDTHGRKERYY
VLYIRPSHIHRRKFDAKGNEIEPNFSATRKVNTGFLMSSYKVEAKGDTDRLTPEALKGLV
NKPELLALTESLTPDHTVAFWMPESEMEVMELELGAGVRLKTRGDGPFLDSLAKLEAGTV
TKCNFTGDGKTGASWTDNIMAQKCSKGAAAEIREQGDGAEDEEWDDC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 773

Start on reference sequence 93

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

C15orf38-AP3S2

Symbol Nomenclature :

C15orf38-AP3S2

Designation :

C15orf38-AP3S2 fusion protein

Full Nomenclature :

C15orf38-AP3S2 readthrough

GENEID :

100526783

HGNC :

38824

Vega :

OTTHUMG00000162161

Target GenBank:

BC053602.1

Reference Sequence Alignment

  

HsCD00288605        ATGAGCCGCATCTACCACGACGGCGCGCTCCGGAACAAGGCGGTGCAGAGCGTCCGGCTG
NM_182616.3         ATGAGCCGCATCTACCACGACGGCGCGCTCCGGAACAAGGCGGTGCAGAGCGTCCGGCTG
NM_001199058.1      ATGAGCCGCATCTACCACGACGGCGCGCTCCGGAACAAGGCGGTGCAGAGCGTCCGGCTG
                    ************************************************************

HsCD00288605        CCAGGGGCCTGGGACCCCGCCGCCCACCAGGGGGGAAATGGTGTCCTGCTGGAGGGAGAA
NM_182616.3         CCAGGGGCCTGGGACCCCGCCGCCCACCAGGGGGGAAATGGTGTCCTGCTGGAGGGAGAA
NM_001199058.1      CCAGGGGCCTGGGACCCCGCCGCCCACCAGGGGGGAAATGGTGTCCTGCTGGAGGGAGAA
                    ************************************************************

HsCD00288605        CTGATCGATGTATCTCGGCACAGCATCTTGGACACTCATGGCAGGAAGGAGCGCTACTAC
NM_182616.3         CTGATCGATGTATCTCGGCACAGCATCTTGGACACTCATGGCAGGAAGGAGCGCTACTAC
NM_001199058.1      CTGATCGATGTATCTCGGCACAGCATCTTGGACACTCATGGCAGGAAGGAGCGCTACTAC
                    ************************************************************

HsCD00288605        GTGCTGTATATCCGGCCCAGTCACATCCATCGCCGTAAATTCGACGCCAAGGGAAATGAA
NM_182616.3         GTGCTGTATATCCGGCCCAGTCACATCCATCGCCGTAAATTCGACGCCAAGGGAAATGAA
NM_001199058.1      GTGCTGTATATCCGGCCCAGTCACATCCATCGCCGTAAATTCGACGCCAAGGGAAATGAA
                    ************************************************************

HsCD00288605        ATCGAGCCCAACTTCAGCGCCACCAGGAAGGTGAACACGGGCTTCCTCATGTCGTCCTAC
NM_182616.3         ATCGAGCCCAACTTCAGCGCCACCAGGAAGGTGAACACGGGCTTCCTCATGTCGTCCTAC
NM_001199058.1      ATCGAGCCCAACTTCAGCGCCACCAGGAAGGTGAACACGGGCTTCCTCATGTCGTCCTAC
                    ************************************************************

HsCD00288605        AAGGTGGAAGCCAAGGGGGACACTGACAGGCTCACGCCCGAGGCGCTGAAGGGGCTGGTC
NM_182616.3         AAGGTGGAAGCCAAGGGGGACACTGACAGGCTCACGCCCGAGGCGCTGAAGGGGCTGGTC
NM_001199058.1      AAGGTGGAAGCCAAGGGGGACACTGACAGGCTCACGCCCGAGGCGCTGAAGGGGCTGGTC
                    ************************************************************

HsCD00288605        AACAAGCCAGAGCTGCTCGCGCTGACAGAGAGCCTCACCCCCGACCACACAGTGGCGTTC
NM_182616.3         AACAAGCCAGAGCTGCTCGCGCTGACAGAGAGCCTCACCCCCGACCACACAGTGGCGTTC
NM_001199058.1      AACAAGCCAGAGCTGCTCGCGCTGACAGAGAGCCTCACCCCCGACCACACAGTGGCGTTC
                    ************************************************************

HsCD00288605        TGGATGCCCGAGTCAGAGATGGAGGTGATGGAACTCGAGCTGGGGGCCGGGGTACGGCTG
NM_182616.3         TGGATGCCCGAGTCAGAGATGGAGGTGATGGAACTCGAGCTGGGGGCCGGGGTACGGCTG
NM_001199058.1      TGGATGCCCGAGTCAGAGATGGAGGTGATGGAACTCGAGCTGGGGGCCGGGGTACGGCTG
                    ************************************************************

HsCD00288605        AAGACTCGGGGCGATGGTCCCTTCCTGGATTCATTGGCCAAACTTGAGGCTGGAACAGTG
NM_182616.3         AAGACTCGGGGCGATGGTCCCTTCCTGGATTCATTGGCCAAACTTGAGGCTGGAACAGTG
NM_001199058.1      AAGACTCGGGGCGATGGTCCCTTCCTGGATTCATTGGCCAAACTTGAGGCTGGAACAGTG
                    ************************************************************

HsCD00288605        ACCAAGTGTAATTTCACTGGTGATGGAAAGACAGGGGCATCCTGGACAGACAACATCATG
NM_182616.3         ACCAAGTGTAATTTCACTGGTGATGGAAAGACAGGGGCATCCTGGACAGACAACATCATG
NM_001199058.1      ACCAAGTGTAATTTCACTGGTGATGGAAAGACAGGGGCATCCTGGACAGACAACATCATG
                    ************************************************************

HsCD00288605        GCCCAAAAGTGTTCGAAGGGGGCTGCAGCGGAGATCCGAGAGCAGGGGGATGGGGCAGAG
NM_182616.3         GCCCAAAAGTGTTCGAAGGGGGCTGCAGCGGAGATCCGAGAGCAGGGGGATGGGGCAGAG
NM_001199058.1      GCCCAAAAGTGTTCGAAGGGGGCTGCAGCGGAGATCCGAGAGCAGGGGGATGGGGCAGAG
                    ************************************************************

HsCD00288605        GACGAGGAGTGGGATGACTGC---------------------------------------
NM_182616.3         GACGAGGAGTGGGATGACTGA---------------------------------------
NM_001199058.1      GACGAGGAGTGGCCAGAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTAGTC
                    ************ .:**. ..                                       

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      CTCAAGCGGGATGACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCT
                                                                                

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      GACTACAAACTGATCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCC
                                                                                

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      TCAGAGAGTGAACTTGGAATCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAG
                                                                                

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      TGTTTCGAAAATGTGTGTGAATTGGATTTGATCTTCCATATGGATAAGGTGCACTACATC
                                                                                

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      CTCCAGGAGGTGGTGATGGGTGGGATGGTGTTGGAAACAAACATGAATGAAATCGTGGCT
                                                                                

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      CAGATTGAGGCTCAAAACAGGCTGGAGAAATCCGAGGGTGGCCTTTCAGCAGCCCCTGCG
                                                                                

HsCD00288605        ------------------------------------------------------------
NM_182616.3         ------------------------------------------------------------
NM_001199058.1      CGGGCTGTGTCTGCTGTGAAAAACATCAACCTGCCAGAGATTCCTCGGAACATCAACATT
                                                                                

HsCD00288605        ---------------------------------------------
NM_182616.3         ---------------------------------------------
NM_001199058.1      GGCGATCTCAACATCAAAGTTCCCAACCTGTCCCAGTTTGTCTGA