DNASU Plasmid | CXCR5 (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: CXCR5

Gene Name: chemokine (C-X-C motif) receptor 5

Original Clone ID:

ATCG3D_24

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

Accelerated Technologies Center for Gene to 3D Structure

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA 2.0 for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Accelerated Technologies Center for Gene to 3D Structure

HsCD00292944

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1218nts

Open reading frame: 7 to 1224

Reference Sequence Alignment

GGATCCATGAAAACTATCATCGCACTGTCTTATATCTTCTGCCTGGTCTTCGCGGACTAC
AAAGACGATGATGATATGAACTATCCACTGACTCTGGAAATGGACCTGGAAAACCTGGAA
GACCTGTTCTGGGAACTGGATCGCCTGGACAACTATAACGATACTAGCCTGGTCGAAAAT
CATCTGTGCCCAGCTACCGAGGGTCCACTGATGGCATCCTTCAAAGCCGTGTTTGTTCCA
GTGGCCTACTCCCTGATTTTCCTGCTGGGTGTTATCGGTAATGTTCTGGTCCTGGTCATC
CTGGAACGTCACCGCCAAACGCGCTCTTCTACCGAAACCTTTCTGTTCCATCTGGCAGTT
GCTGATCTGCTGCTGGTCTTCATTCTGCCATTCGCAGTCGCTGAAGGCTCCGTCGGTTGG
GTCCTGGGTACCTTCCTGTGCAAAACCGTGATCGCCCTGCATAAAGTGAACTTCTATTGC
TCCTCTCTGCTGCTGGCCTGCATCGCTGTTGATCGTTACCTGGCCATTGTGCATGCCGTC
CACGCATACCGCCACCGTCGTCTGCTGTCTATCCACATCACCTGCGGTACTATCTGGCTG
GTGGGCTTTCTGCTGGCTCTGCCGGAAATTCTGTTCGCGAAAGTCTCCCAGGGTCATCAT
AACAACTCCCTGCCTCGTTGCACCTTCAGCCAGGAGAACCAGGCGGAAACCCATGCTTGG
TTTACCTCCCGTTTCCTGTATCATGTGGCCGGTTTCCTGCTGCCAATGCTGGTGATGGGT
TGGTGTTACGTTGGTGTTGTGCACCGCCTGCGTCAAGCCCAGCGCCGTCCGCAGCGTCAG
AAAGCTGTCCGTGTCGCAATCCTGGTGACCTCCATCTTCTTCCTGTGCTGGAGCCCTTAC
CACATTGTCATCTTCCTGGACACCCTGGCCCGTCTGAAAGCGGTCGATAACACTTGTAAG
CTGAACGGCAGCCTGCCGGTTGCGATTACTATGTGCGAATTCCTGGGCCTGGCTCACTGT
TGTCTGAACCCTATGCTGTACACCTTCGCAGGTGTGAAGTTCCGTAGCGATCTGAGCCGT
CTGCTGACCAAGCTGGGCTGCACCGGTCCAGCTTCTCTGTGCCAGCTGTTCCCGAGCTGG
CGTCGCTCTTCCCTGTCTGAATCCGAAAACGCGACTTCTCTGACTACTTTCCACCACCAT
CACCATCACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMNYPLTLEMDLENLEDLFWELDRLDNYNDTSLVENHL
CPATEGPLMASFKAVFVPVAYSLIFLLGVIGNVLVLVILERHRQTRSSTETFLFHLAVAD
LLLVFILPFAVAEGSVGWVLGTFLCKTVIALHKVNFYCSSLLLACIAVDRYLAIVHAVHA
YRHRRLLSIHITCGTIWLVGFLLALPEILFAKVSQGHHNNSLPRCTFSQENQAETHAWFT
SRFLYHVAGFLLPMLVMGWCYVGVVHRLRQAQRRPQRQKAVRVAILVTSIFFLCWSPYHI
VIFLDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLL
TKLGCTGPASLCQLFPSWRRSSLSESENATSLTTFHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_12

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

CXCR5

Symbol Nomenclature :

CXCR5

SYNONYM :

BLR1; CD185; MDR15

Designation :

Full Nomenclature :

chemokine (C-X-C motif) receptor 5

GENEID :

643

HGNC :

1060

MIM :

601613

Target GenBank:

NP_001707

Reference Sequence Alignment

  

HsCD00292944      GGATCCATGAAAACTATCATCGCACTGTCTTATATCTTCTGCCTGGTCTTCGCGGACTAC
NM_001716.4       ------------------------------------------------------------
                                                                              

HsCD00292944      AAAGACGATGATGATATGAACTATCCACTGACTCTGGAAATGGACCTGGAAAACCTGGAA
NM_001716.4       ---------------ATGAACTACCCGCTAACGCTGGAAATGGACCTCGAGAACCTGGAG
                                 ******** **.**.** ************** **.********.

HsCD00292944      GACCTGTTCTGGGAACTGGATCGCCTGGACAACTATAACGATACTAGCCTGGTCGAAAAT
NM_001716.4       GACCTGTTCTGGGAACTGGACAGATTGGACAACTATAACGACACCTCCCTGGTGGAAAAT
                  ******************** .*. **************** ** : ****** ******

HsCD00292944      CATCTGTGCCCAGCTACCGAGGGTCCACTGATGGCATCCTTCAAAGCCGTGTTTGTTCCA
NM_001716.4       CATCTCTGCCCTGCCACAGAGGGGCCCCTCATGGCCTCCTTCAAGGCCGTGTTCGTGCCC
                  ***** *****:** **.***** **.** *****.********.******** ** **.

HsCD00292944      GTGGCCTACTCCCTGATTTTCCTGCTGGGTGTTATCGGTAATGTTCTGGTCCTGGTCATC
NM_001716.4       GTGGCCTACAGCCTCATCTTCCTCCTGGGCGTGATCGGCAACGTCCTGGTGCTGGTGATC
                  *********: *** ** ***** ***** ** ***** ** ** ***** ***** ***

HsCD00292944      CTGGAACGTCACCGCCAAACGCGCTCTTCTACCGAAACCTTTCTGTTCCATCTGGCAGTT
NM_001716.4       CTGGAGCGGCACCGGCAGACACGCAGTTCCACGGAGACCTTCCTGTTCCACCTGGCCGTG
                  *****.** ***** **.**.***: *** ** **.***** ******** *****.** 

HsCD00292944      GCTGATCTGCTGCTGGTCTTCATTCTGCCATTCGCAGTCGCTGAAGGCTCCGTCGGTTGG
NM_001716.4       GCCGACCTCCTGCTGGTCTTCATCTTGCCCTTTGCCGTGGCCGAGGGCTCTGTGGGCTGG
                  ** ** ** **************  ****.** **.** ** **.***** ** ** ***

HsCD00292944      GTCCTGGGTACCTTCCTGTGCAAAACCGTGATCGCCCTGCATAAAGTGAACTTCTATTGC
NM_001716.4       GTCCTGGGGACCTTCCTCTGCAAAACTGTGATTGCCCTGCACAAAGTCAACTTCTACTGC
                  ******** ******** ******** ***** ******** ***** ******** ***

HsCD00292944      TCCTCTCTGCTGCTGGCCTGCATCGCTGTTGATCGTTACCTGGCCATTGTGCATGCCGTC
NM_001716.4       AGCAGCCTGCTCCTGGCCTGCATCGCCGTGGACCGCTACCTGGCCATTGTCCACGCCGTC
                  : *:  ***** ************** ** ** ** ************** ** ******

HsCD00292944      CACGCATACCGCCACCGTCGTCTGCTGTCTATCCACATCACCTGCGGTACTATCTGGCTG
NM_001716.4       CATGCCTACCGCCACCGCCGCCTCCTCTCCATCCACATCACCTGTGGGACCATCTGGCTG
                  ** **.*********** ** ** ** ** ************** ** ** *********

HsCD00292944      GTGGGCTTTCTGCTGGCTCTGCCGGAAATTCTGTTCGCGAAAGTCTCCCAGGGTCATCAT
NM_001716.4       GTGGGCTTCCTCCTTGCCTTGCCAGAGATTCTCTTCGCCAAAGTCAGCCAAGGCCATCAC
                  ******** ** ** **  ****.**.***** ***** ******: ***.** ***** 

HsCD00292944      AACAACTCCCTGCCTCGTTGCACCTTCAGCCAGGAGAACCAGGCGGAAACCCATGCTTGG
NM_001716.4       AACAACTCCCTGCCACGTTGCACCTTCTCCCAAGAGAACCAAGCAGAAACGCATGCCTGG
                  **************:************: ***.********.**.***** ***** ***

HsCD00292944      TTTACCTCCCGTTTCCTGTATCATGTGGCCGGTTTCCTGCTGCCAATGCTGGTGATGGGT
NM_001716.4       TTCACCTCCCGATTCCTCTACCATGTGGCGGGATTCCTGCTGCCCATGCTGGTGATGGGC
                  ** ********:***** ** ******** **:***********.************** 

HsCD00292944      TGGTGTTACGTTGGTGTTGTGCACCGCCTGCGTCAAGCCCAGCGCCGTCCGCAGCGTCAG
NM_001716.4       TGGTGCTACGTGGGGGTAGTGCACAGGTTGCGCCAGGCCCAGCGGCGCCCTCAGCGGCAG
                  ***** ***** ** **:******.*  **** **.******** ** ** ***** ***

HsCD00292944      AAAGCTGTCCGTGTCGCAATCCTGGTGACCTCCATCTTCTTCCTGTGCTGGAGCCCTTAC
NM_001716.4       AAGGCAGTCAGGGTGGCCATCCTGGTGACAAGCATCTTCTTCCTCTGCTGGTCACCCTAC
                  **.**:***.* ** **.***********.: ************ ******: .** ***

HsCD00292944      CACATTGTCATCTTCCTGGACACCCTGGCCCGTCTGAAAGCGGTCGATAACACTTGTAAG
NM_001716.4       CACATCGTCATCTTCCTGGACACCCTGGCGAGGCTGAAGGCCGTGGACAATACCTGCAAG
                  ***** *********************** .* *****.** ** ** ** ** ** ***

HsCD00292944      CTGAACGGCAGCCTGCCGGTTGCGATTACTATGTGCGAATTCCTGGGCCTGGCTCACTGT
NM_001716.4       CTGAATGGCTCTCTCCCCGTGGCCATCACCATGTGTGAGTTCCTGGGCCTGGCCCACTGC
                  ***** ***:  ** ** ** ** ** ** ***** **.************** ***** 

HsCD00292944      TGTCTGAACCCTATGCTGTACACCTTCGCAGGTGTGAAGTTCCGTAGCGATCTGAGCCGT
NM_001716.4       TGCCTCAACCCCATGCTCTACACTTTCGCCGGCGTGAAGTTCCGCAGTGACCTGTCGCGG
                  ** ** ***** ***** ***** *****.** *********** ** ** ***:  ** 

HsCD00292944      CTGCTGACCAAGCTGGGCTGCACCGGTCCAGCTTCTCTGTGCCAGCTGTTCCCGAGCTGG
NM_001716.4       CTCCTGACGAAGCTGGGCTGTACCGGCCCTGCCTCCCTGTGCCAGCTCTTCCCTAGCTGG
                  ** ***** *********** ***** **:** ** *********** ***** ******

HsCD00292944      CGTCGCTCTTCCCTGTCTGAATCCGAAAACGCGACTTCTCTGACTACTTTCCACCACCAT
NM_001716.4       CGCAGGAGCAGTCTCTCTGAGTCAGAGAATGCCACCTCTCTCACCACGTTCTAG------
                  ** .* :  :  ** *****.**.**.** ** ** ***** ** ** *** *       

HsCD00292944      CACCATCACCATCACCATCACTGAAAGCTTCTCGAG
NM_001716.4       ------------------------------------

Pathways

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Reactome :

Chemokine receptors bind chemokines

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

Peptide GPCRs

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

Q2YD84

UniProt :

A0N0R2

UniProt :

A8K647

UniProt :

P32302

HPRD :

03367

Clone

Cloning Information :

485308

HIP Master Clone ID :

401865

Original Clone ID :

ATCG3D_24

Experimental

TargetTrack - Experimental data :

ATCG3D_12

Organism

Species Specific ID:

643

Genome

Chromosome :

Map Location :

11q23.3

Reagents

Labome :

CXCR5-antibody