DNASU Plasmid | TACR1 (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: TACR1

Gene Name: tachykinin receptor 1

Original Clone ID:

ATCG3D_28

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

GPCR Network

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA2.0 for Expression in BV only. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure

HsCD00292957

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1323nts

Open reading frame: 7 to 1329

Reference Sequence Alignment

GGATCCATGAAAACCATTATTGCGTTGAGCTATATATTCTGTCTCGTCTTCGCTGATTAC
AAAGATGACGACGACATGGATAACGTGCTCCCGGTCGATTCTGACCTGAGCCCCAATATT
TCTACAAACACCTCCGAACCCAACCAGTTCGTGCAGCCCGCTTGGCAGATCGTGCTCTGG
GCTGCTGCCTACACCGTCATCGTGGTCACATCCGTCGTCGGTAACGTTGTCGTCATGTGG
ATCATCCTTGCTCACAAGCGTATGAGGACCGTGACAAACTATTTCTTGGTAAATTTGGCT
TTCGCCGAGGCGTCTATGGCCGCTTTCAACACTGTTGTTAACTTCACTTATGCTGTGCAT
AATGAGTGGTATTACGGACTCTTCTACTGCAAATTCCACAATTTCTTCCCGATCGCCGCC
GTGTTCGCTTCCATATATTCCATGACTGCTGTCGCCTTCGACCGTTATATGGCCATTATT
CATCCCCTCCAACCTAGGCTTAGTGCCACCGCTACAAAAGTCGTAATCTGTGTTATCTGG
GTGCTCGCCCTGCTGCTGGCCTTTCCCCAAGGTTACTACTCAACTACCGAGACAATGCCC
TCGCGCGTTGTCTGTATGATCGAATGGCCCGAACACCCGAACAAGATTTACGAGAAGGTA
TACCATATCTGTGTCACAGTTCTGATCTACTTCCTCCCGCTGCTCGTGATCGGCTATGCC
TACACTGTGGTGGGCATCACACTTTGGGCCAGCGAGATTCCTGGTGATTCCTCGGATAGG
TACCACGAGCAAGTGTCCGCGAAGAGAAAGGTAGTCAAGATGATGATCGTAGTCGTCTGT
ACTTTCGCGATCTGCTGGCTCCCATTCCATATCTTCTTTTTGCTTCCATACATCAATCCT
GACCTGTACCTGAAGAAATTCATCCAGCAGGTGTACCTGGCTATCATGTGGCTCGCCATG
TCTTCCACTATGTACAACCCTATCATTTACTGTTGCCTGAACGACCGCTTTCGTCTGGGT
TTCAAGCACGCTTTTAGGTGTTGTCCCTTCATCTCCGCAGGCGACTACGAGGGCCTGGAA
ATGAAGTCTACCCGCTACCTTCAAACCCAGGGCTCTGTGTATAAAGTCTCTCGTTTGGAG
ACTACCATCTCTACCGTGGTCGGAGCTCACGAGGAAGAACCTGAGGACGGTCCAAAGGCT
ACCCCATCATCACTCGACCTGACCTCCAACTGTTCCTCGCGTTCCGACTCTAAGACCATG
ACGGAATCCTTTAGTTTTTCGTCGAACGTACTCAGCCACCACCATCACCATCACCATCAC
CATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMDNVLPVDSDLSPNISTNTSEPNQFVQPAWQIVLWAA
AYTVIVVTSVVGNVVVMWIILAHKRMRTVTNYFLVNLAFAEASMAAFNTVVNFTYAVHNE
WYYGLFYCKFHNFFPIAAVFASIYSMTAVAFDRYMAIIHPLQPRLSATATKVVICVIWVL
ALLLAFPQGYYSTTETMPSRVVCMIEWPEHPNKIYEKVYHICVTVLIYFLPLLVIGYAYT
VVGITLWASEIPGDSSDRYHEQVSAKRKVVKMMIVVVCTFAICWLPFHIFFLLPYINPDL
YLKKFIQQVYLAIMWLAMSSTMYNPIIYCCLNDRFRLGFKHAFRCCPFISAGDYEGLEMK
STRYLQTQGSVYKVSRLETTISTVVGAHEEEPEDGPKATPSSLDLTSNCSSRSDSKTMTE
SFSFSSNVLSHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_4

Find experimental protocols in GPCR-74

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

TACR1

Symbol Nomenclature :

TACR1

SYNONYM :

NK1R; NKIR; SPR; TAC1R

Designation :

NK-1 receptor|NK-1R|substance-P receptor|tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)

Full Nomenclature :

tachykinin receptor 1

GENEID :

6869

HGNC :

11526

MIM :

162323

Vega :

OTTHUMG00000129973

Target GenBank:

NP_001049

Reference Sequence Alignment

  

HsCD00292957      GGATCCATGAAAACCATTATTGCGTTGAGCTATATATTCTGTCTCGTCTTCGCTGATTAC
NM_001058.3       ------------------------------------------------------------
                                                                              

HsCD00292957      AAAGATGACGACGACATGGATAACGTGCTCCCGGTCGATTCTGACCTGAGCCCCAATATT
NM_001058.3       ---------------ATGGATAACGTCCTCCCGGTGGACTCAGACCTCTCCCCAAACATC
                                 *********** ******** ** **:***** : ***.** ** 

HsCD00292957      TCTACAAACACCTCCGAACCCAACCAGTTCGTGCAGCCCGCTTGGCAGATCGTGCTCTGG
NM_001058.3       TCCACTAACACCTCGGAACCCAATCAGTTCGTGCAACCAGCCTGGCAAATTGTCCTTTGG
                  ** **:******** ******** ***********.**.** *****.** ** ** ***

HsCD00292957      GCTGCTGCCTACACCGTCATCGTGGTCACATCCGTCGTCGGTAACGTTGTCGTCATGTGG
NM_001058.3       GCAGCTGCCTACACGGTCATTGTGGTGACCTCTGTGGTGGGCAACGTGGTAGTGATGTGG
                  **:*********** ***** ***** **.** ** ** ** ***** **.** ******

HsCD00292957      ATCATCCTTGCTCACAAGCGTATGAGGACCGTGACAAACTATTTCTTGGTAAATTTGGCT
NM_001058.3       ATCATCTTAGCCCACAAAAGAATGAGGACAGTGACGAACTATTTTCTGGTGAACCTGGCC
                  ****** *:** *****..*:********.*****.********  ****.**  **** 

HsCD00292957      TTCGCCGAGGCGTCTATGGCCGCTTTCAACACTGTTGTTAACTTCACTTATGCTGTGCAT
NM_001058.3       TTCGCGGAGGCCTCCATGGCTGCATTCAATACAGTGGTGAACTTCACCTATGCTGTCCAC
                  ***** ***** ** ***** **:***** **:** ** ******** ******** ** 

HsCD00292957      AATGAGTGGTATTACGGACTCTTCTACTGCAAATTCCACAATTTCTTCCCGATCGCCGCC
NM_001058.3       AACGAATGGTACTACGGCCTGTTCTACTGCAAGTTCCACAACTTCTTTCCCATCGCCGCT
                  ** **.***** *****.** ***********.******** ***** ** ******** 

HsCD00292957      GTGTTCGCTTCCATATATTCCATGACTGCTGTCGCCTTCGACCGTTATATGGCCATTATT
NM_001058.3       GTCTTCGCCAGTATCTACTCCATGACGGCTGTGGCCTTTGATAGGTACATGGCCATCATA
                  ** ***** :  **.** ******** ***** ***** ** .* ** ******** **:

HsCD00292957      CATCCCCTCCAACCTAGGCTTAGTGCCACCGCTACAAAAGTCGTAATCTGTGTTATCTGG
NM_001058.3       CATCCCCTCCAGCCCCGGCTGTCAGCCACAGCCACCAAAGTGGTCATCTGTGTCATCTGG
                  ***********.** .**** : :*****.** **.***** **.******** ******

HsCD00292957      GTGCTCGCCCTGCTGCTGGCCTTTCCCCAAGGTTACTACTCAACTACCGAGACAATGCCC
NM_001058.3       GTCCTGGCTCTCCTGCTGGCCTTCCCCCAGGGCTACTACTCAACCACAGAGACCATGCCC
                  ** ** ** ** *********** *****.** *********** **.*****.******

HsCD00292957      TCGCGCGTTGTCTGTATGATCGAATGGCCCGAACACCCGAACAAGATTTACGAGAAGGTA
NM_001058.3       AGCAGAGTCGTGTGCATGATCGAATGGCCAGAGCATCCGAACAAGATTTATGAGAAAGTG
                  :  .*.** ** ** **************.**.** ************** *****.**.

HsCD00292957      TACCATATCTGTGTCACAGTTCTGATCTACTTCCTCCCGCTGCTCGTGATCGGCTATGCC
NM_001058.3       TACCACATCTGTGTGACTGTGCTGATCTACTTCCTCCCCCTGCTGGTGATTGGCTATGCA
                  ***** ******** **:** ***************** ***** ***** ********.

HsCD00292957      TACACTGTGGTGGGCATCACACTTTGGGCCAGCGAGATTCCTGGTGATTCCTCGGATAGG
NM_001058.3       TACACCGTAGTGGGAATCACACTATGGGCCAGTGAGATCCCCGGGGACTCCTCTGACCGC
                  ***** **.*****.********:******** ***** ** ** ** ***** ** .* 

HsCD00292957      TACCACGAGCAAGTGTCCGCGAAGAGAAAGGTAGTCAAGATGATGATCGTAGTCGTCTGT
NM_001058.3       TACCACGAGCAAGTCTCTGCCAAGCGCAAGGTGGTCAAAATGATGATTGTCGTGGTGTGC
                  ************** ** ** ***.*.*****.*****.******** **.** ** ** 

HsCD00292957      ACTTTCGCGATCTGCTGGCTCCCATTCCATATCTTCTTTTTGCTTCCATACATCAATCCT
NM_001058.3       ACCTTCGCCATCTGCTGGCTGCCCTTCCACATCTTCTTCCTCCTGCCCTACATCAACCCA
                  ** ***** *********** **.***** ********  * ** **.******** **:

HsCD00292957      GACCTGTACCTGAAGAAATTCATCCAGCAGGTGTACCTGGCTATCATGTGGCTCGCCATG
NM_001058.3       GATCTCTACCTGAAGAAGTTTATCCAGCAGGTCTACCTGGCCATCATGTGGCTGGCCATG
                  ** ** ***********.** *********** ******** *********** ******

HsCD00292957      TCTTCCACTATGTACAACCCTATCATTTACTGTTGCCTGAACGACCGCTTTCGTCTGGGT
NM_001058.3       AGCTCCACCATGTACAACCCCATCATCTACTGCTGCCTCAATGACAGGTTCCGTCTGGGC
                  :  ***** *********** ***** ***** ***** ** ***.* ** ******** 

HsCD00292957      TTCAAGCACGCTTTTAGGTGTTGTCCCTTCATCTCCGCAGGCGACTACGAGGGCCTGGAA
NM_001058.3       TTCAAGCATGCCTTCCGGTGCTGCCCCTTCATCAGCGCCGGCGACTATGAGGGGCTGGAA
                  ******** ** ** .**** ** *********: ***.******** ***** ******

HsCD00292957      ATGAAGTCTACCCGCTACCTTCAAACCCAGGGCTCTGTGTATAAAGTCTCTCGTTTGGAG
NM_001058.3       ATGAAATCCACCCGGTATCTCCAGACCCAGGGCAGTGTGTACAAAGTCAGCCGCCTGGAG
                  *****.** ***** ** ** **.*********: ****** ******:  **  *****

HsCD00292957      ACTACCATCTCTACCGTGGTCGGAGCTCACGAGGAAGAACCTGAGGACGGTCCAAAGGCT
NM_001058.3       ACCACCATCTCCACAGTGGTGGGGGCCCACGAGGAGGAGCCAGAGGACGGCCCCAAGGCC
                  ** ******** **.***** **.** ********.**.**:******** **.***** 

HsCD00292957      ACCCCATCATCACTCGACCTGACCTCCAACTGTTCCTCGCGTTCCGACTCTAAGACCATG
NM_001058.3       ACACCCTCGTCCCTGGACCTGACCTCCAACTGCTCTTCACGAAGTGACTCCAAGACCATG
                  **.**.**.**.** ***************** ** **.**::  ***** *********

HsCD00292957      ACGGAATCCTTTAGTTTTTCGTCGAACGTACTCAGCCACCACCATCACCATCACCATCAC
NM_001058.3       ACAGAGAGCTTCAGCTTCTCCTCCAATGTGCTCTCCTAG---------------------
                  **.**.: *** ** ** ** ** ** **.***: * *                      

HsCD00292957      CATCACTGAAAGCTTCTCGAG
NM_001058.3       ---------------------

Pathways

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (q) signalling events

Reactome :

Gastrin-CREB signalling pathway via PKC and MAPK

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

Reactome :

Tachykinin receptors bind tachykinins

WikiPathway :

Peptide GPCRs

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

P25103

HPRD :

01208

Clone

Cloning Information :

485321

HIP Master Clone ID :

401878

Original Clone ID :

ATCG3D_28

Experimental

TargetTrack - Experimental data :

ATCG3D_4

TargetTrack - Experimental data :

GPCR-74

Organism

Species Specific ID:

6869

Genome

Chromosome :

Map Location :

2p12

Ensembl :

ENSG00000115353

Reagents

Labome :

TACR1-antibody