Detailed Information

Clone: EvNO00292962

Explanation of Terms

Clone ID: EvNO00292962 Type: No insert
Is Verified: Y Verification Method: Partial sequencing
Status: NOT AVAILABLE Distribution: Academic and non-profit labs
Source: Center for Structures of Membrane Proteins Special MTA:
Description: Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
Map: pHM3C-LIC vector map.jpg

 

Additional Annotations:

Please note that clicking on the HIP Clone ID will link to additional sequencing information including the linker sequences (the sequences immediately flanking the insert).

 

Collection/Protein Expression Information:

Collection Center for Structures of Membrane Proteins
Collection PSI

 

Insert Information:

Insert Size (bp) Species Mutation Discrepancy Format Tissue Source Species Specific ID Gene Symbol Gene Name Target Genbank Keyword

 

Insert Sequence:

 

Vector Information:

Vector Name: pHM3C-LIC Size (bp): 6528
Type: bacterial plasmid Form: dsDNA
Description: Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Properties: bacterial expression
Comments:
Map: pHM3C-LIC vector map.jpg
Sequence: HM3C.doc

 

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y T1/T5 phage resistant DH5-alpha

 

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

 

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

 

Authors:

Author Name Author Type

 

Publications:

PMID Title