Gene:
Gene Symbol:
SCAPER
Gene Name:
S-phase cyclin A-associated protein in the ER
Original Clone ID:
FLH269942.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297174
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTTCATTCCAGCGCTCCAATAGTCATGACAAAGTAAGGAGAATAGTTGCAGAGGAG
GGTCGTACAGCAAGAAACCTAATAGCTTGGAGTGTTCCACTAGAAAGCAAAGATGATGAT
GGAAAACCTAAATGTCAAACTGGTGGAAAATCTAAGAGGACCATTCAAGGCACTCATAAA
ACTACTAAACAGAGTACTGCAGTGGACTGTAAAATAACATCGTCTACGACTGGAGATAAA
CACTTTGATAAAAGTCCCACTAAAACAAGGCACCCTCGGAAAATTGATCTAAGAGCTCGA
TACTGGGCATTTCTTTTTGATAATCTTCGCCGAGCAGTAGATGAAATCTATGTAACTTGC
GAATCAGATCAGAGTGTGGTCGAATGTAAGGAGGTGCTAATGATGCTGGATAACTATGTA
AGAGATTTCAAAGCATTGATTGACTGGATTCAGCTTCAGGAAAAGCTAGAGAAGACAGAT
GCTCAAAGCAGACCAACATCATTGGCATGGGAAGTAAAGAAGATGTCTCCGGGACGCCAT
GTGATTCCAAGTCCATCAACAGATAGAATAAATGTAACATCAAATGCTCGACGAAGCTTA
AATTTTGGAGGTTCAACTGGCACAGTGCCAGCTCCTCGTCTGGCTCCCACAGGTGTCAGT
TGGGCTGACAAGGTAAAGGCTCATCATACAGGCTCTACTGCTTCTTCAGAAATAACACCC
GCCCAGTCTTGCCCACCAATGACAGTGCAGAAGGCCTCACGCAAAAATGAACGGAAAGAT
GCTGAAGGATGGGAGACCGTTCAGAGAGGAAGGCCTATTCGTTCTCGATCAACAGCAGTG
ATGCCAAAAGTTTCATTGGCAACAGAAGCCACAAGATCAAAGGATGACAGTGATAAAGAA
AATGTATGTCTTTTACCTGATGAAAGCATACAGAAAGGTCAATTTGTTGGAGATGGAACT
TCTAATACTATAGAATCTCATCCCAAAGACTCATTACACTCTTGTGACCATCCTCTTGCC
GAAAAAACCCAGTTCACAGTGAGTACATTGGATGATGTGAAGAATTCTGGCAGTATTCGA
GACAATTATGTTCGAACTTCTGAAATATCTGCTGTCCACATTGATACAGAGTGTGTTTCA
GTTATGCTGCAAGCTGGTACACCTCCTTTACAAGTAAATGAAGAAAAATTTCCAGCAGAG
AAAGCAAGGATAGAAAATGAAATGGACCCTTCAGATATTTCAAATTCCATGGCAGAAGTC
CTTGCTAAAAAAGAAGAGCTAGCAGATCGTCTAGAAAAGGCCAATGAAGAAGCCATTGCT
AGTGCTATTGCTGAAGAAGAACAGTTAACTAGAGAAATTGAAGCTGAAGAAAACAATGAT
ATTAACATTGAAACTGACAACGACAGTGATTTTTCTGCCAGCATGGGCAGTGGGAGTGTT
TCTTTCTGTGGTATGTCCATGGACTGGAACGATGTCCTTGCAGATTATGAAGCTCGTGAG
TCTTGGCGCCAAAATACATCCTGGGGGGACATTGTAGAAGAAGAACCTGCTAGACCTCCA
GGGCATGGAATTCACATGCATGAAAAACTTTCTTCACCCTCTCGTAAAAGAACAATTGCA
GAATCTAAGAAGAAACATGAAGAAAAACAAATGAAAGCACAGCAGCTAAGGGAAAAGTTA
CGCGAAGAGAAAACATTGAAGCTTCAGAAATTGTTAGAAAGGGAGAAGGATGTCCGGAAG
TGGAAGGAAGAATTGCTAGATCAACGACGCAGGATGATGGAAGAAAAATTACTTCATGCT
GAGTTTAAGCGAGAAGTGCAGTTACAAGCAATTGTGAAAAAAGCACAAGAAGAAGAAGCT
AAGGTAAATGAAATTGCCTTTATAAATACCCTTGAAGCCCAGAATAAACGTCATGATGTT
TTATCAAAATTGAAGGAATATGAACAGAGGCTTAATGAGCTACAGGAAGAGCGTCAGAGA
AGACAGGAAGAAAAGCAAGCACGTGATGAAGCTGTGCAGGAACGCAAGAGAGCTCTAGAG
GCAGAGCGGCAGGCCCGTGTAGAAGAATTGTTAATGAAGAGGAAAGAACAAGAAGCCCGA
ATTGAACAACAGAGGCAAGAAAAGGAAAAAGCCCGTGAGGATGCAGCCCGGGAAAGAGCT
AGAGACAGGGAAGAACGATTGGCAGCACTCACAGCTGCTCAACAAGAAGCTATGGAAGAG
TTACAGAAAAAAATTCAGCTCAAGCATGATGAAAGTATTCGAAGGCACATGGAACAGATT
GAACAAAGAAAAGAAAAAGCTGCTGAGCTAAGCAGTGGGCGACATGCAAATACTGATTAT
GCCCCCAAACTGACCCCTTATGAAAGAAAGAAGCAGTGTTCTCTCTGCAATGTCCTGATC
TCTTCAGAGGTATATCTTTTTAGCCATGTTAAAGGGAGAAAACACCAGCAAGCCGTGAGA
GAGAATACCAGCATCCAGGGGCGTGAACTGTCAGATGAAGAAGTGGAGCATCTTTCCTTG
AAGAAGTACATTATTGACATTGTGGTTGAAAGTACAGCTCCAGCAGAAGCTTTGAAAGAT
GGAGAAGAGCGGCAAAAAAATAAAAAAAAAGCCAAAAAGATAAAAGCCCGGATGAACTTC
AGGGCTAAGGAATATGAGAGTTTAATGGAAACCAAAAATTCTGGCTCTGATTCACCTTAT
AAAGCAAAGCTTCAGCGATTAGCCAAAGATCTTCTAAAACAAGTACAAGTTCAAGACAGT
GGCTCATGGGCAAACAATAAAGTGTCTGCTTTGGATCGGACCCTAGGAGAGATCACTAGA
ATACTGGAAAAAGAGAATGTGGCAGATCAGATTGCATTTCAAGCTGCTGGTGGATTAACA
GCCCTTGAACACATCCTTCAAGCAGTAGTCCCAGCCACAAATGTGAACACAGTTTTAAGA
ATTCCTCCTAAGTCTCTCTGCAATGCAATCAATGTTTACAACCTCACCTGCAATAACTGT
TCAGAAAACTGCAGTGATGTTCTGTTTAGTAACAAGATTACCTTCTTAATGGACCTCCTG
ATACACCAGTTGACGGTTTATGTTCCAGATGAAAATAATACTATTTTGGGGAGAAATACA
AATAAACAAGTTTTTGAAGGCTTGACAACTGGACTTCTCAAAGTCAGTGCTGTGGTTTTG
GGCTGCCTGATTGCCAATCGACCAGATGGAAACTGCCAGCCAGCTACCCCAAAAATACCA
ACACAGGAAATGAAAAACAAACCCTCACAAGGTGATCCTTTTAACAATCGAGTTCAGGAC
CTTATCAGCTACGTGGTGAACATGGGTCTGATTGACAAACTGTGTGCCTGCTTCCTCTCG
GTGCAAGGCCCAGTGGATGAGAATCCCAAGATGGCCATATTTCTGCAGCATGCCGCAGGA
CTCTTACATGCAATGTGTACACTGTGCTTTGCTGTCACTGGAAGGTCATACAGCATATTT
GACAATAATCGCCAGGATCCCACAGGGCTGACAGCTGCTCTTCAGGCAACCGACCTGGCT
GGAGTTCTTCATATGCTCTACTGTGTCCTCTTCCATGGCACCATCTTGGACCCCAGCACT
GCCAGTCCCAAGGAGAATTACACTCAAAATACCATCCAAGTGGCCATTCAGAGTTTACGT
TTCTTCAACAGCTTTGCAGCTCTTCATCTGCCTGCTTTTCAGTCTATTGTAGGGGCAGAG
GGCTTGTCCCTTGCATTCCGGCACATGGCCAGCTCCCTGCTGGGCCACTGCAGCCAAGTC
TCCTGTGAAAGCCTCCTTCATGAGGTCATCGTCTGTGTGGGCTACTTCACTGTCAACCAC
CCAGATAACCAGGTGATCGTGCAGTCCGGCCGCCACCCCACAGTGCTGCAGAAGCTCTGC
CAGTTGCCCTTCCAGTATTTCAGTGACCCACGGCTGATCAAAGTACTGTTCCCTTCACTT
ATCGCTGCTTGTTACAACAACCATCAGAACAAGATCATTCTGGAGCAAGAGATGAGCTGT
GTTTTACTGGCCACTTTCATTCAGGATTTGGCACAGACTCCAGGTCAAGCGGAAAACCAG
CCTTACCAACCCAAAGGGAAATGCCTTGGTTCCCAAGACTATCTTGAGCTGGCTAACAGA
TTTCCTCAGCAGGCCTGGGAAGAAGCTCGACAGTTTTTCTTGAAAAAAGAGAAAAAA
Protein sequence predicted based on nucleotide sequence
MASFQRSNSHDKVRRIVAEEGRTARNLIAWSVPLESKDDDGKPKCQTGGKSKRTIQGTHK TTKQSTAVDCKITSSTTGDKHFDKSPTKTRHPRKIDLRARYWAFLFDNLRRAVDEIYVTC ESDQSVVECKEVLMMLDNYVRDFKALIDWIQLQEKLEKTDAQSRPTSLAWEVKKMSPGRH VIPSPSTDRINVTSNARRSLNFGGSTGTVPAPRLAPTGVSWADKVKAHHTGSTASSEITP AQSCPPMTVQKASRKNERKDAEGWETVQRGRPIRSRSTAVMPKVSLATEATRSKDDSDKE NVCLLPDESIQKGQFVGDGTSNTIESHPKDSLHSCDHPLAEKTQFTVSTLDDVKNSGSIR DNYVRTSEISAVHIDTECVSVMLQAGTPPLQVNEEKFPAEKARIENEMDPSDISNSMAEV LAKKEELADRLEKANEEAIASAIAEEEQLTREIEAEENNDINIETDNDSDFSASMGSGSV SFCGMSMDWNDVLADYEARESWRQNTSWGDIVEEEPARPPGHGIHMHEKLSSPSRKRTIA ESKKKHEEKQMKAQQLREKLREEKTLKLQKLLEREKDVRKWKEELLDQRRRMMEEKLLHA EFKREVQLQAIVKKAQEEEAKVNEIAFINTLEAQNKRHDVLSKLKEYEQRLNELQEERQR RQEEKQARDEAVQERKRALEAERQARVEELLMKRKEQEARIEQQRQEKEKAREDAARERA RDREERLAALTAAQQEAMEELQKKIQLKHDESIRRHMEQIEQRKEKAAELSSGRHANTDY APKLTPYERKKQCSLCNVLISSEVYLFSHVKGRKHQQAVRENTSIQGRELSDEEVEHLSL KKYIIDIVVESTAPAEALKDGEERQKNKKKAKKIKARMNFRAKEYESLMETKNSGSDSPY KAKLQRLAKDLLKQVQVQDSGSWANNKVSALDRTLGEITRILEKENVADQIAFQAAGGLT ALEHILQAVVPATNVNTVLRIPPKSLCNAINVYNLTCNNCSENCSDVLFSNKITFLMDLL IHQLTVYVPDENNTILGRNTNKQVFEGLTTGLLKVSAVVLGCLIANRPDGNCQPATPKIP TQEMKNKPSQGDPFNNRVQDLISYVVNMGLIDKLCACFLSVQGPVDENPKMAIFLQHAAG LLHAMCTLCFAVTGRSYSIFDNNRQDPTGLTAALQATDLAGVLHMLYCVLFHGTILDPST ASPKENYTQNTIQVAIQSLRFFNSFAALHLPAFQSIVGAEGLSLAFRHMASSLLGHCSQV SCESLLHEVIVCVGYFTVNHPDNQVIVQSGRHPTVLQKLCQLPFQYFSDPRLIKVLFPSL IAACYNNHQNKIILEQEMSCVLLATFIQDLAQTPGQAENQPYQPKGKCLGSQDYLELANR FPQQAWEEARQFFLKKEKK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
38
End on reference sequence
4237
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
