48835872 (T. fusca YX )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9312b1BCt3p1
Keyword:
full-length cds
Species:
Thermobifida fusca YX
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 24060347
Title: Divergent Evolution of Ligand Binding in the o-Succinylbenzoate Synthase Family.
Authors:
PSI
New York SGX Research Center for Structural Genomics
TfCD00336395
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 975nts
Open reading frame: 1 to 975

ACTGGCAGAGCGTTTGCCATTCCGCTGCGCACGCGTTTCCGTGGTATCACTGTGCGTGAA
GGTATGTTAGTTCGCGGTGCAGCTGGTTGGGGTGAGTTTAGCCCATTCGCAGAATATGGC
CCTCGGGAATGCGCTCGTTGGTGGGCAGCTTGTTACGAAGCGGCAGAACTGGGTTGGCCC
GCTCCGGTACGTGATACAGTTCCAGTAAATGCCACTGTCCCTGCAGTTGGCCCGGAAGAA
GCTGCACGTATTGTGGCTTCTAGTGGGTGTACCACGGCGAAAGTCAAGGTGGCCGAACGC
GGTCAGTCAGAAGCAAACGACGTCGCTCGTGTAGAGGCAGTTCGCGACGCTCTGGGTCCC
CGTGGTCGTGTACGTATCGATGTTAATGGCGCGTGGGATGTTGACACAGCCGTACGCATG
ATTCGCTTGCTTGACCGCTTTGAGCTCGAGTATGTGGAGCAACCGTGCGCGACCGTTGAT
GAGTTAGCCGAAGTGAGACGGCGCGTCTCCGTACCAATAGCGGCCGATGAGTCTATCCGA
AGAGCGGAGGACCCTTTGCGGGTAAGAGATGCAGAAGCCGCTGATGTTGTGGTACTTAAA
GTCCAGCCGCTGGGCGGTGTTCGAGCAGCTCTGCGTCTCGCTGAGGAATGTGGACTCCCC
GTAGTTGTGTCGAGTGCGGTTGAAACGAGCGTCGGTCTGGCTGCAGGTGTAGCTCTGGCA
GCTGCACTGCCGGAACTGCCATACGCTTGTGGTCTGGCAACTCTGCGTCTGCTGCATGCT
GATGTTTGTGATGACCCTCTGCTGCCGGTACACGGTGTTCTGCCAGTACGTCGTGTTGAT
GTGTCAGAGCAACGCTTAGCCGAAGTGGAAATAGACCCGGCCGCTTGGCAAGCACGTCTG
GCAGCTGCACGTGCTGCATGGGAACAAGTAGAACGCGAACCCGGTCCTGAAGGGCATCAT
CACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

TGRAFAIPLRTRFRGITVREGMLVRGAAGWGEFSPFAEYGPRECARWWAACYEAAELGWP
APVRDTVPVNATVPAVGPEEAARIVASSGCTTAKVKVAERGQSEANDVARVEAVRDALGP
RGRVRIDVNGAWDVDTAVRMIRLLDRFELEYVEQPCATVDELAEVRRRVSVPIAADESIR
RAEDPLRVRDAEAADVVVLKVQPLGGVRAALRLAEECGLPVVVSSAVETSVGLAAGVALA
AALPELPYACGLATLRLLHADVCDDPLLPVHGVLPVRRVDVSEQRLAEVEIDPAAWQARL
AAARAAWEQVEREPGPEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t348c,D116D;a549g,E183E;
g564a,V188V;c565a,c567a,R189R;
g639c,L213L;a642t,A214A;
a645g,E215E;c856t,g858a,L286L;
a861c,A287A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9312b
Recommended expression in:
Not Applicable