Gene:
Gene Symbol:
ALG3
Gene Name:
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase
Original Clone ID:
YBL082C_pSGP36
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pSGP36
Format:
FUSION
Source:
Center for High-Throughput Structural Biology
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Rochester
Center for High Throughput Structural Biology
PSI
Center for High Throughput Structural Biology
PSI
ScCD00343118
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1374nts
Open reading frame: 1 to 1374
ATGGAAGGTGAACAGTCTCCGCAAGGTGAAAAGTCTCTGCAAAGGAAGCAATTTGTCAGA
CCTCCGCTGGATCTGTGGCAGGATCTCAAGGACGGTGTGCGCTACGTGATCTTCGATTGT
AGGGCCAATCTTATCGTTATGCCCCTTTTGATTTTGTTCGAAAGCATGCTGTGCAAGATT
ATCATTAAGAAGGTAGCTTACACAGAGATCGATTACAAGGCGTACATGGAGCAGATCGAG
ATGATTCAGCTCGATGGCATGCTGGACTACTCTCAGGTGAGTGGTGGAACGGGCCCGCTG
GTGTATCCAGCAGGCCACGTCTTGATCTACAAGATGATGTACTGGCTAACAGAGGGAATG
GACCACGTTGAGCGCGGGCAAGTGTTTTTCAGATACTTGTATCTCCTTACACTGGCGTTA
CAAATGGCGTGTTACTACCTTTTACATCTACCACCGTGGTGTGTGGTCTTGGCGTGCCTC
TCTAAAAGATTGCACTCTATTTACGTGCTACGGTTATTCAATGATTGCTTCACTACTTTG
TTTATGGTCGTCACGGTTTTGGGGGCTATCGTGGCCAGCAGGTGCCATCAGCGCCCCAAA
TTAAAGAAGTCCCTTGCGCTGGTGATCTCCGCAACATACAGTATGGCTGTGAGCATTAAG
ATGAATGCGCTGTTGTATTTCCCTGCAATGATGATTTCTCTATTCATCCTTAATGACGCG
AACGTAATCCTTACTTTGTTGGATCTCGTTGCGATGATTGCATGGCAAGTCGCAGTTGCA
GTGCCCTTCCTGCGCAGCTTTCCGCAACAGTACCTGCATTGCGCTTTTAATTTCGGCAGG
AAGTTTATGTACCAATGGAGTATCAATTGGCAAATGATGGATGAAGAGGCTTTCAATGAT
AAGAGGTTCCACTTGGCCCTTTTAATCAGCCACCTGATAGCGCTCACCACACTGTTCGTC
ACAAGATACCCTCGCATCCTGCCCGATTTATGGTCTTCCCTGTGCCATCCGCTGAGGAAA
AATGCAGTGCTCAATGCCAATCCCGCCAAGACTATTCCATTCGTTCTAATCGCATCCAAC
TTCATCGGCGTCCTATTTTCAAGGTCCCTCCACTACCAGTTTCTATCCTGGTATCACTGG
ACTTTGCCTATACTGATCTTTTGGTCGGGAATGCCCTTCTTCGTTGGTCCCATTTGGTAC
GTCTTGCACGAGTGGTGCTGGAATTCCTATCCACCAAACTCACAAGCAAGCACGCTATTG
TTGGCATTGAATACTGTTCTGTTGCTTCTATTGGCCTTGACGCAGCTATCTGGTTCGGTC
GCCCTCGCCAAAAGCCATCTTCGTACCACCAGCTCTATGGAAAAAAAGCTCAAC
Protein sequence predicted based on nucleotide sequence
MEGEQSPQGEKSLQRKQFVRPPLDLWQDLKDGVRYVIFDCRANLIVMPLLILFESMLCKI IIKKVAYTEIDYKAYMEQIEMIQLDGMLDYSQVSGGTGPLVYPAGHVLIYKMMYWLTEGM DHVERGQVFFRYLYLLTLALQMACYYLLHLPPWCVVLACLSKRLHSIYVLRLFNDCFTTL FMVVTVLGAIVASRCHQRPKLKKSLALVISATYSMAVSIKMNALLYFPAMMISLFILNDA NVILTLLDLVAMIAWQVAVAVPFLRSFPQQYLHCAFNFGRKFMYQWSINWQMMDEEAFND KRFHLALLISHLIALTTLFVTRYPRILPDLWSSLCHPLRKNAVLNANPAKTIPFVLIASN FIGVLFSRSLHYQFLSWYHWTLPILIFWSGMPFFVGPIWYVLHEWCWNSYPPNSQASTLL LALNTVLLLLLALTQLSGSVALAKSHLRTTSSMEKKLN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
URA
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in YBL082C
Recommended expression in:
Not Applicable
Vector Name:
pSGP36
Synonyms:
pSGP-36
Sequencing Primer:
Forward:
pSGP36 forward
Reverse:
pSGP36 reverse
Description:
Yeast expression vector with Gal1 promoter, adds C-terminal 3C-his10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
Comments:
None
Size (bp):
6185
Parent Vector:
None
Properties:
inducible expression, ligation independent cloning (LIC), with tag/fusion/marker, yeast expression, yeast shuttle
Author Name:
Center for High Throughput Structural Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1 bacterial origin
2499
3181
primer
forward
CAACATTTTCAGTTTGTATTACTTC
3983
4007
primer
reverse
CAAGGCGATTAAGTTGGGTAACGC
4279
4302
promoter
Ura3 promoter
Ura3 promoter
5870
5899
promoter
T7
T7 promoter
4209
4229
protease cleavage site
3C
3C protease cleavage site
4122
4145
reporter gene
LacZ
LacZ alpha gene
4326
4394
selectable marker
AmpR
ampicillin resistance gene
1742
2401
selectable marker
Ura3
URA3 nutritional marker
4981
5784
tag
His
10xHis tag
4164
4193
trxn regulatory element
lac operator
lac operator
3521
3543
