Original Clone ID:
NCI10392
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer
HsCD00343160
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
ATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAAGACAATCTGGGAGGTG
CCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTCTGTGTGTGCT
GCTTTTGACACAAAAACGGGGTTACGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAG
TCCATCATTCATGCGAAAAGAACCTACAGAGAACTGCGGTTACTTAAACATATGAAACAT
GAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAAGGTCTCTGGAGGAATTCAAT
GATGTGTATCTGGTGACCCATCTCATGGGGGCAGATCTGAACAACATTGTGAAATGTCAG
AAGCTTACAGATGACCATGTTCAGTTCCTTATCTACCAAATTCTCCGAGGTCTAAAGTAT
ATACATTCAGCTGACATAATTCACAGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAA
GACTGTGAGCTGAAGATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACA
GGCTACGTGGCCACTAGGTGGTACAGGGCTCCTGAGATCATGCTGAACTGGATGCATTAC
AACCAGACAGTTGATATTTGGTCAGTGGGATGCATAATGGCCGAGCTGTTGACTGGAAGA
ACATTGTTTCCTGGTACAGACCATATTGATCAGTTGAAGCTCATTTTAAGACTCGTTGGA
ACCCCAGGGGCTGAGCTTTTGAAGAAAATCTCCTCAGAGTCTGCAAGAAACTATATTCAG
TCTTTGACTCAGATGCCGAAGATGAACTTTGCGAATGTATTTATTGGTGCCAATCCCCTG
GCTGTCGACTTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACAGCGGCC
CAAGCCCTTGCACATGCCTACTTTGCTCAGTACCACGATCCTGATGATGAACCAGTGGCC
GATCCTTATGATCAGTCCTTTGAAAGCAGGGACCTCCTTATAGATGAGTGGAAAAGCCTG
ACCTATGATGAAGTCATCAGCTTTGTGCCACCACCCCTTGACCAAGAAGAGATGGAGTCC
TGA
Protein sequence predicted based on nucleotide sequence
MSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQ SIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQ KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMT GYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVG TPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAA QALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES *
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Confirmed
SolubleProtein: Protein_Confirmed
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
Gene
Gene Symbol :
MAPK14
Symbol Nomenclature :
MAPK14
SYNONYM :
CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA
Designation :
CSAID-binding protein|Csaids binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|cytokine suppressive anti-inflammatory drug-binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A
Full Nomenclature :
mitogen-activated protein kinase 14
GENEID :
Locus Tag :
RP1-179N16.5
GI :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Reference Sequence Alignment
HsCD00343160 ATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAAGACAATCTGGGAGGTG NM_139012.2 ATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAAGACAATCTGGGAGGTG NM_001315.2 ATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAAGACAATCTGGGAGGTG ************************************************************ HsCD00343160 CCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTCTGTGTGTGCT NM_139012.2 CCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTCTGTGTGTGCT NM_001315.2 CCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTCTGTGTGTGCT ************************************************************ HsCD00343160 GCTTTTGACACAAAAACGGGGTTACGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAG NM_139012.2 GCTTTTGACACAAAAACGGGGTTACGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAG NM_001315.2 GCTTTTGACACAAAAACGGGGTTACGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAG ************************************************************ HsCD00343160 TCCATCATTCATGCGAAAAGAACCTACAGAGAACTGCGGTTACTTAAACATATGAAACAT NM_139012.2 TCCATCATTCATGCGAAAAGAACCTACAGAGAACTGCGGTTACTTAAACATATGAAACAT NM_001315.2 TCCATCATTCATGCGAAAAGAACCTACAGAGAACTGCGGTTACTTAAACATATGAAACAT ************************************************************ HsCD00343160 GAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAAGGTCTCTGGAGGAATTCAAT NM_139012.2 GAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAAGGTCTCTGGAGGAATTCAAT NM_001315.2 GAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAAGGTCTCTGGAGGAATTCAAT ************************************************************ HsCD00343160 GATGTGTATCTGGTGACCCATCTCATGGGGGCAGATCTGAACAACATTGTGAAATGTCAG NM_139012.2 GATGTGTATCTGGTGACCCATCTCATGGGGGCAGATCTGAACAACATTGTGAAATGTCAG NM_001315.2 GATGTGTATCTGGTGACCCATCTCATGGGGGCAGATCTGAACAACATTGTGAAATGTCAG ************************************************************ HsCD00343160 AAGCTTACAGATGACCATGTTCAGTTCCTTATCTACCAAATTCTCCGAGGTCTAAAGTAT NM_139012.2 AAGCTTACAGATGACCATGTTCAGTTCCTTATCTACCAAATTCTCCGAGGTCTAAAGTAT NM_001315.2 AAGCTTACAGATGACCATGTTCAGTTCCTTATCTACCAAATTCTCCGAGGTCTAAAGTAT ************************************************************ HsCD00343160 ATACATTCAGCTGACATAATTCACAGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAA NM_139012.2 ATACATTCAGCTGACATAATTCACAGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAA NM_001315.2 ATACATTCAGCTGACATAATTCACAGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAA ************************************************************ HsCD00343160 GACTGTGAGCTGAAGATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACA NM_139012.2 GACTGTGAGCTGAAGATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACA NM_001315.2 GACTGTGAGCTGAAGATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACA ************************************************************ HsCD00343160 GGCTACGTGGCCACTAGGTGGTACAGGGCTCCTGAGATCATGCTGAACTGGATGCATTAC NM_139012.2 GGCTACGTGGCCACTAGGTGGTACAGGGCTCCTGAGATCATGCTGAACTGGATGCATTAC NM_001315.2 GGCTACGTGGCCACTAGGTGGTACAGGGCTCCTGAGATCATGCTGAACTGGATGCATTAC ************************************************************ HsCD00343160 AACCAGACAGTTGATATTTGGTCAGTGGGATGCATAATGGCCGAGCTGTTGACTGGAAGA NM_139012.2 AACCAGACAGTTGATATTTGGTCAGTGGGATGCATAATGGCCGAGCTGTTGACTGGAAGA NM_001315.2 AACCAGACAGTTGATATTTGGTCAGTGGGATGCATAATGGCCGAGCTGTTGACTGGAAGA ************************************************************ HsCD00343160 ACATTGTTTCCTGGTACAGACCATATTGATCAGTTGAAGCTCATTTTAAGACTCGTTGGA NM_139012.2 ACATTGTTTCCTGGTACAGACCATATTGATCAGTTGAAGCTCATTTTAAGACTCGTTGGA NM_001315.2 ACATTGTTTCCTGGTACAGACCATATTAACCAGCTTCAGCAGATTATGCGTCTGACAGGA ***************************.* *** * .***: ***:*..*:** . :*** HsCD00343160 ACCCCAGGGGCTGAGCTTTTGAAGAAAATCTCCTCAGAGTCTGCAAGAAACTATATTCAG NM_139012.2 ACCCCAGGGGCTGAGCTTTTGAAGAAAATCTCCTCAGAGTCTGCAAGAAACTATATTCAG NM_001315.2 ACACCCCCCGCTTATCTCATTAACAGGATGCCAAGCCATGAGGCAAGAAACTATATTCAG **.**. *** * ** :* ** *..** *.: . * . ****************** HsCD00343160 TCTTTGACTCAGATGCCGAAGATGAACTTTGCGAATGTATTTATTGGTGCCAATCCCCTG NM_139012.2 TCTTTGACTCAGATGCCGAAGATGAACTTTGCGAATGTATTTATTGGTGCCAATCCCCTG NM_001315.2 TCTTTGACTCAGATGCCGAAGATGAACTTTGCGAATGTATTTATTGGTGCCAATCCCCTG ************************************************************ HsCD00343160 GCTGTCGACTTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACAGCGGCC NM_139012.2 GCTGTCGACTTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACAGCGGCC NM_001315.2 GCTGTCGACTTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACAGCGGCC ************************************************************ HsCD00343160 CAAGCCCTTGCACATGCCTACTTTGCTCAGTACCACGATCCTGATGATGAACCAGTGGCC NM_139012.2 CAAGCCCTTGCACATGCCTACTTTGCTCAGTACCACGATCCTGATGATGAACCAGTGGCC NM_001315.2 CAAGCCCTTGCACATGCCTACTTTGCTCAGTACCACGATCCTGATGATGAACCAGTGGCC ************************************************************ HsCD00343160 GATCCTTATGATCAGTCCTTTGAAAGCAGGGACCTCCTTATAGATGAGTGGAAAAGCCTG NM_139012.2 GATCCTTATGATCAGTCCTTTGAAAGCAGGGACCTCCTTATAGATGAGTGGAAAAGCCTG NM_001315.2 GATCCTTATGATCAGTCCTTTGAAAGCAGGGACCTCCTTATAGATGAGTGGAAAAGCCTG ************************************************************ HsCD00343160 ACCTATGATGAAGTCATCAGCTTTGTGCCACCACCCCTTGACCAAGAAGAGATGGAGTCC NM_139012.2 ACCTATGATGAAGTCATCAGCTTTGTGCCACCACCCCTTGACCAAGAAGAGATGGAGTCC NM_001315.2 ACCTATGATGAAGTCATCAGCTTTGTGCCACCACCCCTTGACCAAGAAGAGATGGAGTCC ************************************************************ HsCD00343160 TGA NM_139012.2 TGA NM_001315.2 TGA ***
Pathways
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
ATF-2 transcription factor network
NCI :
Atypical NF-kappaB pathway
NCI :
BCR signaling pathway
NCI :
CD40/CD40L signaling
NCI :
CDC42 signaling events
NCI :
CXCR3-mediated signaling events
NCI :
Endothelins
NCI :
EPO signaling pathway
NCI :
FAS (CD95) signaling pathway
NCI :
FGF signaling pathway
NCI :
Glucocorticoid receptor regulatory network
NCI :
IL12-mediated signaling events
NCI :
IL2-mediated signaling events
NCI :
IL4-mediated signaling events
NCI :
IL6-mediated signaling events
NCI :
p38 MAPK signaling pathway
NCI :
p38 signaling mediated by MAPKAP kinases
NCI :
p53 pathway
NCI :
p73 transcription factor network
NCI :
RAC1 signaling pathway
NCI :
Rapid glucocorticoid signaling
NCI :
Regulation of Androgen receptor activity
NCI :
Regulation of p38-alpha and p38-beta
NCI :
Regulation of retinoblastoma protein
NCI :
Retinoic acid receptors-mediated signaling
NCI :
Role of Calcineurin-dependent NFAT signaling in lymphocytes
NCI :
S1P2 pathway
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Signaling mediated by p38-alpha and p38-beta
NCI :
Thromboxane A2 receptor signaling
NCI :
Trk receptor signaling mediated by the MAPK pathway
NCI :
VEGFR3 signaling in lymphatic endothelium
Panther :
Alzheimer disease-amyloid secretase pathway
Panther :
Angiogenesis
Panther :
B cell activation
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Interferon-gamma signaling pathway
Panther :
Oxidative stress response
Panther :
p38 MAPK pathway
Panther :
p53 pathway feedback loops 2
Panther :
Parkinson disease
Panther :
Ras Pathway
Panther :
TGF-beta signaling pathway
Panther :
Toll receptor signaling pathway
Panther :
VEGF signaling pathway
Reactome :
activated TAK1 mediates p38 MAPK activation
Reactome :
Activated TLR4 signalling
Reactome :
Activation of PPARGC1A (PGC-1alpha) by phosphorylation
Reactome :
Activation of the AP-1 family of transcription factors
Reactome :
ADP signalling through P2Y purinoceptor 1
Reactome :
CDO in myogenesis
Reactome :
Cell-Cell communication
Reactome :
Cellular responses to stress
Reactome :
Cellular Senescence
Reactome :
Developmental Biology
Reactome :
DSCAM interactions
Reactome :
ERK/MAPK targets
Reactome :
Gene Expression
Reactome :
Hemostasis
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
KSRP destabilizes mRNA
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MAPK targets/ Nuclear events mediated by MAP kinases
Reactome :
Metabolism of mRNA
Reactome :
Metabolism of RNA
Reactome :
Mitochondrial biogenesis
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
Myogenesis
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
NOD1/2 Signaling Pathway
Reactome :
Nuclear Events (kinase and transcription factor activation)
Reactome :
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways
Reactome :
Organelle biogenesis and maintenance
Reactome :
Oxidative Stress Induced Senescence
Reactome :
p38MAPK events
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Platelet homeostasis
Reactome :
Platelet sensitization by LDL
Reactome :
Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome :
Signal amplification
Reactome :
Signal Transduction
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to RAS
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Energy Metabolism
WikiPathway :
Epithelium TarBase
WikiPathway :
Estrogen signaling pathway
WikiPathway :
FSH signaling pathway
WikiPathway :
IL-1 signaling pathway
WikiPathway :
IL-4 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
Leukocyte TarBase
WikiPathway :
Lymphocyte TarBase
WikiPathway :
MAPK Cascade
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
Monoamine Transport
WikiPathway :
Muscle cell TarBase
WikiPathway :
Oxidative Stress
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Physiological and Pathological Hypertrophy of the Heart
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
Senescence and Autophagy
WikiPathway :
Serotonin HTR1 Group and FOS Pathway
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TSH signaling pathway
WikiPathway :
TWEAK Signaling Pathway
Protein
SMART domain :
SMART domain :
UniProt :
HPRD :
02619
Clone
Organism
Species Specific ID:
Genome
Reagents
Labome :