Rsph17025_0377 (C. sphaeroides ATCC 17025 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Rsph17025_0377
Gene Name:  membrane dipeptidase
Original Clone ID:
9523c1BCt14p1
Keyword:
None
Species:
Cereibacter sphaeroides ATCC 17025
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
RsCD00344782
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1086nts
Open reading frame: 1 to 1086

AGCGACACGATCCCCGTCTTCGACGGACACAACGACTTCCTCCTGCGCCTCCTGCGCAAT
CCCGCCAACCGCGAGACGATCTGGCTGAAGGGCGACGGCACCGGCCATCTCGACCTGCCG
CGGATGAAGGAGGGCGGCTTTGCGGGCGGCTTCTTCGCCATCTACGTCCCCTCGCCGCAG
GCCCACGATGCCGCGCATTTCGAGGCGATGATGGACGCTCCGCCCTTCGAACTGCCGCTG
CCCCCGATGATCCGGGCCGAGCAGGCGCAGCCCGTGGCCCTCGCCATGGCGGGGCACCTG
CTCTGGATGGAACGCGCGGCCCGCGGCCGCTTCAAGGTCTGCCGCACGGCGGCCGAGGTC
CGGTCCTGCCATGCCGACGGCATCGTCTCGGGCATCATGCATATGGAGGGCGCCGAGGCG
ATCGGGGCGGATCTCGATGCGCTCCACCTCTTCCATTCGCTGGGCCTGCGCTCGCTCGGC
CCGGTCTGGAGCCGCCCCACGGTGTTCGGCCACGGCGTGCCCTTCCGCTTCCCCGGCTCG
CCCGATACCGGCGAGGGGCTGACCGAGGCGGGGCGTCGCCTCGTGGCCGAATGCAACCGG
CTGAAGATCATGCTCGACCTCTCGCACCTGAACGAAAAGGGCTTCGACGATGTCGCGCGC
CTGAGCGACGCGCCGCTGGTCGCGACCCATTCCAACGCCCATGCGGTCACGCCCTCGACC
CGCAACCTGACCGACCGCCAGCTCGCGATGATCCGCGAGAGCCGGGGCATGGTGGGGCTG
AATTTCGCCACCTCCTTCCTGCGCGAGGACGGCCGACGCTCGGCCGAGATGGGCTGGGAG
CCGGTGCTGCGCCATCTCGATCATCTGATCGACCGGCTGGGCGAGGATCATGTGGGGATG
GGCTCGGACTTCGACGGGGCCACGATCCCGCAGGGCATCGCCGACGTGACCGGCCTGCCC
GCGCTGCAGGCCGCGATGCGCGCCCATGGCTACGACGAGCCGCTGATGCGCAAGCTCTGC
CACGAGAACTGGTACGGGCTGCTGGAGCGCACCTGGGGGGAAGGGCATCATCACCATCAC
CACTGA

Protein sequence predicted based on nucleotide sequence

SDTIPVFDGHNDFLLRLLRNPANRETIWLKGDGTGHLDLPRMKEGGFAGGFFAIYVPSPQ
AHDAAHFEAMMDAPPFELPLPPMIRAEQAQPVALAMAGHLLWMERAARGRFKVCRTAAEV
RSCHADGIVSGIMHMEGAEAIGADLDALHLFHSLGLRSLGPVWSRPTVFGHGVPFRFPGS
PDTGEGLTEAGRRLVAECNRLKIMLDLSHLNEKGFDDVARLSDAPLVATHSNAHAVTPST
RNLTDRQLAMIRESRGMVGLNFATSFLREDGRRSAEMGWEPVLRHLDHLIDRLGEDHVGM
GSDFDGATIPQGIADVTGLPALQAAMRAHGYDEPLMRKLCHENWYGLLERTWGEGHHHHH
H*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
33 total mis-matches, first 20: R31K; A45E; I57V; S65A; D67H; N73D; N74A; D78E; A83P; L84M; H87A; V118A; D120E; L121V; E127D; T181S; S186E; D190E; Q213E; E217D

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9523c
Recommended expression in:
Not Applicable