CHEK2 (Homo sapiens ) in pMCSG19 (MBP and His-tagged expression vector)
| Gene: |
Gene Symbol:
CHEK2 Gene Name: CHK2 checkpoint homolog (S. pombe) Sequence |
HsCD00358147 Price: Login for Pricing AVAILABLE No restriction Special MTA: SPTA |
|
|---|---|---|---|
| Original Clone ID: | NCI10395 | ||
| Keyword: | None | ||
| Species: | Homo sapiens | ||
| Type: | cDNA | ||
| Vector Name: | pMCSG19 Format: CLOSED | ||
| Source: | Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer | ||
| Description: | None | ||
| Comments: | None | ||
| Mutation/ Discrepancy : |
No / No | ||
| Publications: | None | ||
| Authors: |
Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer |
Insert sequence: 1632nts
Open reading frame : 1 to 1632
ATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAG
CCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGC
TCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTG
AGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCT
GAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAG
GATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGC
TGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGC
AAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATA
GAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGC
CGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTC
TTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATAC
ATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGG
AAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCA
GCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTA
AATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTT
TTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAA
GAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAA
AACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAG
GACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTC
ATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGG
ACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGC
CTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATC
ACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTG
GACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCC
TTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCT
GAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAG
CGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCT
GCTGTGTTGTGA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Mutations: None |
Discrepancy: None |
5' Linker Sequence:
TCCAATGCC |
|
| 3' Linker Sequence: None | |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
| Protein Expression Results: |
ProteinExpressed: Protein_Confirmed ProteinPurified: Not_Tested SolubleProtein: Tested_Not_Soluble |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pMCSG19 | |
| Synonyms: | pMCSG-19 | |
| Description: | Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). | |
| Comments: | pMCSG7 derivative: in vivo TVMV cleavage of MBP | |
| Size (bp): | 6441 | |
| Parent Vector: | None | |
| Empty Vector: | EvNO00084265 | |
| Properties: | bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker | |
| Author Name: |
MCSG |
|
| Publications: |
PMID:
16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins PMID: 18988021 Title: A family of LIC vectors for high-throughput cloning and purification of proteins. |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| MCS | LIC | Ligation independent cloning (LIC) site | 204 | 235 |
| bacterial origin | ori | ColE1-type bacterial origin of replication | 4416 | 5098 |
| promoter | T7 | T7 promoter | 1515 | 1531 |
| protease cleavage site | TVMV | TVMV protease cleavage site | 286 | 306 |
| protease cleavage site | TEV | TEV cleavage site cds | 223 | 243 |
| repressor binding site | lac operator | LacO fragment | 1490 | 1512 |
| repressor protein gene | LacI | LacI coding sequence | 2045 | 3004 |
| selectable marker | AmpR | ampicillin resistance gene | 5193 | 6053 |
| tag | His 2 | C-terminal 6xHis tag | 140 | 157 |
| tag | His | 6xHis tag | 268 | 285 |
| tag | MBP | N-terminal MBP | 337 | 1443 |
| trxn termination sequence | T7 term | T7 terminator sequence | 26 | 72 |
| Cloning Information: |
566173 |
|---|---|
| GI: |
13278894 |
| GenBank Accession: |
BC004207 |
| HIP Master Clone ID: |
476491 |
| Labome: |
CHK2-antibody-review |
| Labome: |
CHK2-antibody |
| Original Clone ID: |
NCI10395 |
| UniProt: |
O96017 |
| Species Specific ID: | 11200 |
| Target GenBank: | BC004207 |