tolC (F. tularensis ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  tolC
Gene Name:  outer membrane protein TolC
Original Clone ID:
MPID027_FTT_1724c_F
Keyword:
None
Species:
Francisella tularensis
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Center for Membrane Proteins of Infectious Diseases
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Arizona State University
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
FtCD00413457
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1530nts
Open reading frame: 1 to 1530

ATGAAGAAGTTAACATTATATCTTTTAGGTTTTTTGGGGTTTTGTCTAGCTTTAGCTAAT
GAAGATACGCCTGTTCCTGAATATAAGTATGCAGGAAGACCTATTGGTACTGATGTTGCT
GAAAATCCATATAGTGTCGAAAAACAATATGCCAAAGCAGATCAAATTAAGGTAGACAAT
AAATTCTATAATCTTAAAAAAGCTGATGAAGTCGCGATGGTTGGCGATACTGAGGACAAG
TATTATAGTTTAATTGATATCTATAAGTTGGCTGCTGAACATAATGCCGACTACCAAGCA
GCAAGGTCTACTTTTGCGGCAAATGTTGAAACTGTGCCGACTGCTTTAGGTGCATTATTA
CCACAGATTGATTTTAACTATAATCTAAGAAGAGATTTGTACAATCAGTTTGGTGGTAGA
GTCAATGACACTTCAAATGTAGTAAATTTTAGTGGTTCTCAGGTTCTTTTTGATTGGAGT
AAATGGAAGACATATACTCAAGCAACCTATTTGCAAAAATCGTATGCGATGATTTATGCT
AAAGCTGAACAAACACTTATAACTGATACTGTTACTGCATATTTTGAGCTGCTAAGAGCA
GAACAGGCGTTACAGTTCCAATTTGCAAATGAGGCTTGGAACAAAAAGTTATATCTGACT
CAGAAATATCAGTATAATTCGGGCATGGTTTCATATGCTGATTTTAAAACTACAGATGCA
CAATATCGTCAAGCAATCGCTGATAGGGTTAATGCACAAAGAAACTTCATTAATGCCAAA
GCTGTGATGGCTAAACTTATCGGTAAGCGTATAAGCTCAATTTTATATATATCAAAAGAT
ACTGAATTTGGTAATCCTGTTCCAGATGATATTAACTATTGGTTGAATACAGCTGAGAAA
TATAATCTTGATATAGCGCAGAAAAAGTTTGAATATGAAGCAGCACAAGAAGGTGTTGGT
ATTCAATGGGGTAAATTTTTTCCTCAAGCTAATTTAACTGGCGGTATAATGATGTCACGT
AACGCTCTTTCGGGTTCACCAGCACAAATAGCCGAAATTCCAACCAAATATGACGTTGCT
AATATCGGTGGTCAAGTTAGCTGGAATTTATTAAGAGGTGGTTCTGACTATGCGCAACTC
AAGCAAGCTAGTTATGATAATCAAGCGGCGAACTATGCCTTGCTACAAACCAAGAGAGAA
GTCTACGCTGGTACAGTAGAGGCATATCAAACAATTGTTCTTGATGTAGTTAGAATTGAG
GCGTTTAGAAAGTCTGTGTATTCAGGTATAGCTTCTGTAAAAGCGATATTAGAAGGTTTT
GAAGCAGGTACACAGACAATTGTTGACTTACTTAACCGTCAAGCAATCCTTGTCCAAGCA
CAATTATCATTTGCAGATGCTATATTCAACTATGTTGAAGATTATGTAAGTTTAAAGCAA
TTACAGGGGAGTTTAACCTACAAAGACATTGAGTACATCAATACTATTTTAGGAACTACA
GATATAATATCGCAGATTGCAACGGAGTTG

Protein sequence predicted based on nucleotide sequence

MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDN
KFYNLKKADEVAMVGDTEDKYYSLIDIYKLAAEHNADYQAARSTFAANVETVPTALGALL
PQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWSKWKTYTQATYLQKSYAMIYA
KAEQTLITDTVTAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEK
YNLDIAQKKFEYEAAQEGVGIQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVA
NIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKREVYAGTVEAYQTIVLDVVRIE
AFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATEL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
S91A, I191V, V412I. A416V

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable