Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9252h1BCt12p1
Keyword:
full-length cds
Species:
Unknown
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 18442260
Title: A common catalytic mechanism for proteins of the HutI family
Authors:
PSI
New York SGX Research Center for Structural Genomics
UnCD00430274
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1242nts
Open reading frame: 51 to 1292

TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAATTGCGAAC
GTGTGTGGCTGAACGTTACCCCGGCGACGTTACGCAGCGATTTGGCCGACTATGGCCTTC
TCGAGCCACATGCATTAGGGGTCCACGAAGGGCGTATTCATGCTCTGGTACCTATGCAGG
ATTTAAAAGGACCCTACCCGGCGCACTGGCAAGACATGAAGGGCAAATTGGTGACTCCAG
GTCTTATCGATTGTCATACACACCTGATATTTGCCGGGTCTCGGGCAGAGGAATTTGAGC
TCCGCCAGAAAGGAGTTCCTTATGCTGAGATTGCGAGAAAGGGCGGTGGCATCATTAGTA
CCGTCCGAGCCACGCGTGCAGCTTCAGAAGACCAACTGTTCGAATTGGCGTTGCCGCGCG
TAAAGTCCTTGATCCGGGAAGGTGTGACTACAGTTGAAATAAAATCGGGGTATGGTCTGA
CCCTGGAGGATGAACTCAAGATGTTACGTGTCGCCCGCAGACTGGGCGAGGCTCTCCCAA
TTCGCGTTAAAACGACCCTTCTGGCTGCGCATGCCGTACCGCCAGAATATCGTGATGACC
CTGATAGCTGGGTTGAAACTATCTGCCAGGAGATTATACCGGCAGCGGCTGAAGCCGGTC
TTGCTGATGCGGTGGATGTATTCTGTGAGCACATCGGCTTTTCTCTGGCTCAGACAGAAC
AAGTTTACCTCGCCGCAGACCAGTACGGATTAGCGGTAAAAGGGCATATGGACCAATTAA
GTAATCTTGGAGGCTCAACGCTGGCTGCCAACTTCGGTGCATTATCCGTGGATCACCTGG
AATACCTCGACCCCGAGGGGATTCAGGCGTTGGCCCATCGCGGCGTCGTTGCAACCCTGC
TTCCGACTGCGTTTTATTTCTTAAAGGAAACAAAACTGCCACCTGTGGTAGCTCTGCGGA
AAGCCGGTGTCCCGATGGCGGTTTCGAGCGATATCAATCCAGGAACCGCTCCGATTGTGT
CTCTCCGTATGGCAATGAACATGGCCTGCACGTTGTTTGGCCTTACCCCCGTAGAAGCGA
TGGCTGGTGTTACTCGCCACGCAGCCCGTGCTCTTGGTGAGCAGGAACAACTGGGCCAGC
TGCGCGTGGGTATGTTAGCCGACTTCTTGGTCTGGAATTGTGGACATCCTGCAGAGTTAA
GTTATCTGATAGGCGTTGATCAGCTTGTGTCAAGAGTAGTCAACGGTGAAGAAACACTGC
ATGGGGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCG

Protein sequence predicted based on nucleotide sequence

NCERVWLNVTPATLRSDLADYGLLEPHALGVHEGRIHALVPMQDLKGPYPAHWQDMKGKL
VTPGLIDCHTHLIFAGSRAEEFELRQKGVPYAEIARKGGGIISTVRATRAASEDQLFELA
LPRVKSLIREGVTTVEIKSGYGLTLEDELKMLRVARRLGEALPIRVKTTLLAAHAVPPEY
RDDPDSWVETICQEIIPAAAEAGLADAVDVFCEHIGFSLAQTEQVYLAADQYGLAVKGHM
DQLSNLGGSTLAANFGALSVDHLEYLDPEGIQALAHRGVVATLLPTAFYFLKETKLPPVV
ALRKAGVPMAVSSDINPGTAPIVSLRMAMNMACTLFGLTPVEAMAGVTRHAARALGEQEQ
LGQLRVGMLADFLVWNCGHPAELSYLIGVDQLVSRVVNGEETLHGEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c85t,g87a,L29L;t90g,G30G;
a249g,E83E;t351c,F117F;
g354a,E118E;a357g,L119L;
c361t,L121L;a375g,K125K;
c423t,Y141Y;a426t,G142G;
t429g,L143L;a483t,A161A;
t484c,g486c,L162L;c489a,P163P;
a495c,R165R;g498t,V166V;
t610c,a612t,L204L;a615t,A205A;
c618t,D206D;c630a,V210V;
t696c,Y232Y;t699a,G233G;
c700t,g702a,L234L;t723c,D241D;
g729a,L243L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9252h
Recommended expression in:
Not Applicable