DNASU Plasmid | IPPK (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: IPPK

Gene Name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase

Original Clone ID:

ccsbBroadEn_03963

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC026154.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515840

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1474nts

Open reading frame: 1 to 1474

Reference Sequence Alignment

ATGGAAGAGGGGAAGATGGACGAGAATGAATGGGGGTACCACGGAGAGGGCAATAAGAGC
CTGGTGGTGGCCCACGCGCAGCGCTGCGTCGTGCTGCGGTTTCTGAAGTTTCCTCCAAAT
AGGAAGAAGACCTCGGAAGAGATATTTCAACACCTGCAGAACATAGTGGACTTTGGGAAA
AATGTCATGAAGGAGTTTTTGGGGGAGAACTATGTTCATTATGGGGAGGTCGTTCAGCTA
CCTTTAGAGTTTGTGAAACAGCTTTGTTTAAAGATACAATCTGAAAGACCAGAGTCTCGC
TGTGACAAGGACCTGGATACTCTCAGTGGTTACGCTATGTGCCTTCCTAATTTAACCAGA
CTCCAAACCTACCGCTTTGCAGAGCACCGGCCGATTCTGTGTGTAGAGATTAAGCCAAAA
TGTGGGTTTATTCCTTTCTCGAGTGATGTCACGCATGAGATGAAGCATAAGGTCTGTCGA
TACTGCATGCACCAGCACCTCAAGGTAGCAACTGGGAAGTGGAAGCAGATCAGCAAATAC
TGTCCCCTTGATCTCTACTCAGGAAACAAACAGAGAATGCACTTTGCCTTGAAGAGTTTG
CTGCAGGAGGCACAGAACAACTTGAAGATATTTAAGAATGGTGAGCTGATTTACGGCTGC
AAAGATGCCCGGAGCCCCGTGGCTGACTGGAGCGAGCTTGCACACCACCTGAAGCCGTTC
TTCTTCCCTTCCAACGGCCTGGCCAGTGGGCCCCACTGCACAAGGGCTGTGATCAGGGAG
CTGGTGCACGTGATCACACGGGTGCTGCTGAGTGGCTCGGACAAGGGCCGGGCAGGCACC
CTGAGTCCGGGGCTCGGGCCTCAGGGCCCGCGAGTCTGCGAAGCCAGCCCTTTCAGTAGG
AGCCTTCGCTGCCAAGGAAAAAACACCCCAGAGCGCTCGGGGTTACCGAAGGGCTGTCTT
CTGTACAAAACCCTCCAGGTGCAGATGTTGGACCTGCTGGACATCGAAGGCCTCTACCCT
CTGTACAACCGGGTTGAGCGATACCTGGAAGAGTTTCCCGAGGAGAGAAAAACCTTACAA
ATAGATGGGCCTTATGATGAAGCATTTTACCAGAAGCTGCTTGACCTTTCCACTGAGGAT
GACGGGACAGTGGCCTTCGCGCTAACGAAGGTGCAGCAGTACCGCGTCGCCATGACTGCC
AAGGACTGCTCCATCATGATTGCACTGTCTCCCTGTCTGCAGGATGCCAGCTCTGATCAA
AGGCCTGTCGTCCCTTCATCGAGGTCCAGGTTTGCCTTTTCCGTGTCTGTGCTGGACCTT
GACCTCAAGCCCTACGAGAGCATTCCCCATCAGTATAAACTGGACGGCAAGATCGTCAAC
TATTATTCAAAGACTGTACGTGCCAAAGACAACGCCGTGATGTCGACTCGGTTCAAGGAA
AGCGAAGATTGCACATTAGTTCTCCACAAGGTC

Protein sequence predicted based on nucleotide sequence

MEEGKMDENEWGYHGEGNKSLVVAHAQRCVVLRFLKFPPNRKKTSEEIFQHLQNIVDFGK
NVMKEFLGENYVHYGEVVQLPLEFVKQLCLKIQSERPESRCDKDLDTLSGYAMCLPNLTR
LQTYRFAEHRPILCVEIKPKCGFIPFSSDVTHEMKHKVCRYCMHQHLKVATGKWKQISKY
CPLDLYSGNKQRMHFALKSLLQEAQNNLKIFKNGELIYGCKDARSPVADWSELAHHLKPF
FFPSNGLASGPHCTRAVIRELVHVITRVLLSGSDKGRAGTLSPGLGPQGPRVCEASPFSR
SLRCQGKNTPERSGLPKGCLLYKTLQVQMLDLLDIEGLYPLYNRVERYLEEFPEERKTLQ
IDGPYDEAFYQKLLDLSTEDDGTVAFALTKVQQYRVAMTAKDCSIMIALSPCLQDASSDQ
RPVVPSSRSRFAFSVSVLDLDLKPYESIPHQYKLDGKIVNYYSKTVRAKDNAVMSTRFKE
SEDCTLVLHKV

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

IPPK

Symbol Nomenclature :

IPPK

SYNONYM :

C9orf12; INSP5K2; IP5K; IPK1; bA476B13.1

Designation :

IPK1 homolog|bA476B13.1 (novel protein)|inositol-pentakisphosphate 2-kinase|ins(1,3,4,5,6)P5 2-kinase|insP5 2-kinase

Full Nomenclature :

inositol 1,3,4,5,6-pentakisphosphate 2-kinase

GENEID :

64768

HGNC :

14645

Vega :

OTTHUMG00000020231

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515840      ATGGAAGAGGGGAAGATGGACGAGAATGAATGGGGGTACCACGGAGAGGGCAATAAGAGC
NM_022755.5       ATGGAAGAGGGGAAGATGGACGAGAATGAATGGGGGTACCACGGAGAGGGCAATAAGAGC
                  ************************************************************

HsCD00515840      CTGGTGGTGGCCCACGCGCAGCGCTGCGTCGTGCTGCGGTTTCTGAAGTTTCCTCCAAAT
NM_022755.5       CTGGTGGTGGCCCACGCGCAGCGCTGCGTCGTGCTGCGGTTTCTGAAGTTTCCTCCAAAT
                  ************************************************************

HsCD00515840      AGGAAGAAGACCTCGGAAGAGATATTTCAACACCTGCAGAACATAGTGGACTTTGGGAAA
NM_022755.5       AGGAAGAAGACCTCGGAAGAGATATTTCAACACCTGCAGAACATAGTGGACTTTGGGAAA
                  ************************************************************

HsCD00515840      AATGTCATGAAGGAGTTTTTGGGGGAGAACTATGTTCATTATGGGGAGGTCGTTCAGCTA
NM_022755.5       AATGTCATGAAGGAGTTTTTGGGGGAGAACTATGTTCATTATGGGGAGGTCGTTCAGCTA
                  ************************************************************

HsCD00515840      CCTTTAGAGTTTGTGAAACAGCTTTGTTTAAAGATACAATCTGAAAGACCAGAGTCTCGC
NM_022755.5       CCTTTAGAGTTTGTGAAACAGCTTTGTTTAAAGATACAATCTGAAAGACCAGAGTCTCGC
                  ************************************************************

HsCD00515840      TGTGACAAGGACCTGGATACTCTCAGTGGTTACGCTATGTGCCTTCCTAATTTAACCAGA
NM_022755.5       TGTGACAAGGACCTGGATACTCTCAGTGGTTACGCTATGTGCCTTCCTAATTTAACCAGA
                  ************************************************************

HsCD00515840      CTCCAAACCTACCGCTTTGCAGAGCACCGGCCGATTCTGTGTGTAGAGATTAAGCCAAAA
NM_022755.5       CTCCAAACCTACCGCTTTGCAGAGCACCGGCCGATTCTGTGTGTAGAGATTAAGCCAAAA
                  ************************************************************

HsCD00515840      TGTGGGTTTATTCCTTTCTCGAGTGATGTCACGCATGAGATGAAGCATAAGGTCTGTCGA
NM_022755.5       TGTGGGTTTATTCCTTTCTCGAGTGATGTCACGCATGAGATGAAGCATAAGGTCTGTCGA
                  ************************************************************

HsCD00515840      TACTGCATGCACCAGCACCTCAAGGTAGCAACTGGGAAGTGGAAGCAGATCAGCAAATAC
NM_022755.5       TACTGCATGCACCAGCACCTCAAGGTAGCAACTGGGAAGTGGAAGCAGATCAGCAAATAC
                  ************************************************************

HsCD00515840      TGTCCCCTTGATCTCTACTCAGGAAACAAACAGAGAATGCACTTTGCCTTGAAGAGTTTG
NM_022755.5       TGTCCCCTTGATCTCTACTCAGGAAACAAACAGAGAATGCACTTTGCCTTGAAGAGTTTG
                  ************************************************************

HsCD00515840      CTGCAGGAGGCACAGAACAACTTGAAGATATTTAAGAATGGTGAGCTGATTTACGGCTGC
NM_022755.5       CTGCAGGAGGCACAGAACAACTTGAAGATATTTAAGAATGGTGAGCTGATTTACGGCTGC
                  ************************************************************

HsCD00515840      AAAGATGCCCGGAGCCCCGTGGCTGACTGGAGCGAGCTTGCACACCACCTGAAGCCGTTC
NM_022755.5       AAAGATGCCCGGAGCCCCGTGGCTGACTGGAGCGAGCTTGCACACCACCTGAAGCCGTTC
                  ************************************************************

HsCD00515840      TTCTTCCCTTCCAACGGCCTGGCCAGTGGGCCCCACTGCACAAGGGCTGTGATCAGGGAG
NM_022755.5       TTCTTCCCTTCCAACGGCCTGGCCAGTGGGCCCCACTGCACAAGGGCTGTGATCAGGGAG
                  ************************************************************

HsCD00515840      CTGGTGCACGTGATCACACGGGTGCTGCTGAGTGGCTCGGACAAGGGCCGGGCAGGCACC
NM_022755.5       CTGGTGCACGTGATCACACGGGTGCTGCTGAGTGGCTCGGACAAGGGCCGGGCAGGCACC
                  ************************************************************

HsCD00515840      CTGAGTCCGGGGCTCGGGCCTCAGGGCCCGCGAGTCTGCGAAGCCAGCCCTTTCAGTAGG
NM_022755.5       CTGAGTCCGGGGCTCGGGCCTCAGGGCCCGCGAGTCTGCGAAGCCAGCCCTTTCAGTAGG
                  ************************************************************

HsCD00515840      AGCCTTCGCTGCCAAGGAAAAAACACCCCAGAGCGCTCGGGGTTACCGAAGGGCTGTCTT
NM_022755.5       AGCCTTCGCTGCCAAGGAAAAAACACCCCAGAGCGCTCGGGGTTACCGAAGGGCTGTCTT
                  ************************************************************

HsCD00515840      CTGTACAAAACCCTCCAGGTGCAGATGTTGGACCTGCTGGACATCGAAGGCCTCTACCCT
NM_022755.5       CTGTACAAAACCCTCCAGGTGCAGATGTTGGACCTGCTGGACATCGAAGGCCTCTACCCT
                  ************************************************************

HsCD00515840      CTGTACAACCGGGTTGAGCGATACCTGGAAGAGTTTCCCGAGGAGAGAAAAACCTTACAA
NM_022755.5       CTGTACAACCGGGTTGAGCGATACCTGGAAGAGTTTCCCGAGGAGAGAAAAACCTTACAA
                  ************************************************************

HsCD00515840      ATAGATGGGCCTTATGATGAAGCATTTTACCAGAAGCTGCTTGACCTTTCCACTGAGGAT
NM_022755.5       ATAGATGGGCCTTATGATGAAGCATTTTACCAGAAGCTGCTTGACCTTTCCACTGAGGAT
                  ************************************************************

HsCD00515840      GACGGGACAGTGGCCTTCGCGCTAACGAAGGTGCAGCAGTACCGCGTCGCCATGACTGCC
NM_022755.5       GACGGGACAGTGGCCTTCGCGCTAACGAAGGTGCAGCAGTACCGCGTCGCCATGACTGCC
                  ************************************************************

HsCD00515840      AAGGACTGCTCCATCATGATTGCACTGTCTCCCTGTCTGCAGGATGCCAGCTCTGATCAA
NM_022755.5       AAGGACTGCTCCATCATGATTGCACTGTCTCCCTGTCTGCAGGATGCCAGCTCTGATCAA
                  ************************************************************

HsCD00515840      AGGCCTGTCGTCCCTTCATCGAGGTCCAGGTTTGCCTTTTCCGTGTCTGTGCTGGACCTT
NM_022755.5       AGGCCTGTCGTCCCTTCATCGAGGTCCAGGTTTGCCTTTTCCGTGTCTGTGCTGGACCTT
                  ************************************************************

HsCD00515840      GACCTCAAGCCCTACGAGAGCATTCCCCATCAGTATAAACTGGACGGCAAGATCGTCAAC
NM_022755.5       GACCTCAAGCCCTACGAGAGCATTCCCCATCAGTATAAACTGGACGGCAAGATCGTCAAC
                  ************************************************************

HsCD00515840      TATTATTCAAAGACTGTACGTGCCAAAGACAACGCCGTGATGTCGACTCGGTTCAAGGAA
NM_022755.5       TATTATTCAAAGACTGTACGTGCCAAAGACAACGCCGTGATGTCGACTCGGTTCAAGGAA
                  ************************************************************

HsCD00515840      AGCGAAGATTGCACATTAGTTCTCCACAAGGTC---
NM_022755.5       AGCGAAGATTGCACATTAGTTCTCCACAAGGTCTAA
                  *********************************

Pathways

Reactome :

Inositol phosphate metabolism

Reactome :

Metabolism

Reactome :

Synthesis of IPs in the nucleus

Reactome :

Synthesis of pyrophosphates in the cytosol

Protein

HPRD :

10799

Clone

Cloning Information :

670041

Original Clone ID :

ccsbBroadEn_03963

Organism

Species Specific ID:

64768

Genome

Chromosome :

Map Location :

9q22.31

Ensembl :

ENSG00000127080

Reagents

Labome :

1-3-4-5-6-pentakisphosphate-2-kinase-antibody