DNASU Plasmid | RAB11FIP4 (H. sapiens ) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RAB11FIP4

Gene Name: RAB11 family interacting protein 4 (class II)

Original Clone ID:

ccsbBroadEn_09188

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC101517.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515989

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1912nts

Open reading frame: 1 to 1912

Reference Sequence Alignment

ATGGCGGGCGGCGCGGGCTGGTCGGGCGCCCCCGCGGCTCTGCTGCGCTCCGTGCGCCGC
CTGCGCGAGGTGTTCGAGGTGTGCGGCCGCGACCCCGACGGCTTCCTGCGCGTGGAGCGC
GTCGCGGCGCTCGGACTGCGCTTCGGCCAGGGCGAGGAGGTGGAAAAACTTGTGAAATAT
TTGGATCCCAACGACCTGGGGAGAATCAACTTCAAGGACTTTTGCCGGGGGGTGTTCGCC
ATGAAAGGGTGCGAGGAGCTGCTGAAGGATGTGCTGTCGGTGGAGAGCGCGGGGACGCTG
CCGTGCGCGCCAGAGATCCCAGACTGCGTGGAGCAGGGCAGCGAGGTCACAGGCCCCACC
TTTGCTGATGGCGAGCTCATCCCCAGGGAACCCGGCTTTTTTCCCGAGGACGAGGAGGAG
GCTATGACGCTGGCGCCACCTGAGGGCCCCCAGGAGTTGTACACAGACAGCCCCATGGAG
AGCACTCAGAGCCTGGAGGGGTCTGTCGGGAGTCCTGCCGAGAAGGACGGGGGACTTGGG
GGCCTGTTTCTGCCAGAAGACAAGTCCCTGGTCCACACTCCATCCATGACGACCTCAGAC
CTTTCTACACACTCCACCACCTCGCTCATCAGCAATGAGGAGCAGTTTGAAGACTATGGG
GAGGGTGACGATGTGGACTGTGCCCCCAGCAGCCCTTGCCCCGATGATGAGACCAGGACC
AACGTCTACTCGGACCTGGGGTCTTCGGTGTCTTCCAGTGCGGGGCAGACGCCTAGGAAA
ATGCGGCACGTGTACAACAGCGAATTGCTAGATGTTTACTGCTCTCAATGCTGCAAGAAA
ATCAACCTGCTCAATGACTTGGAAGCCCGACTGAAAAACCTGAAGGCCAACAGCCCCAAC
CGAAAGATCTCCAGCACGGCCTTTGGACGGCAGCTCATGCACAGCAGCAACTTCAGCAGC
AGCAATGGCAGCACCGAAGACCTGTTCCGGGACAGCATTGACTCTTGCGACAATGACATC
ACAGAGAAGGTAAGCTTCCTGGAAAAGAAGGTGACAGAGCTGGAGAATGACAGCCTGACC
AATGGGGACCTGAAGAGCAAGCTGAAGCAAGAGAACACACAGCTGGTGCACAGGGTGCAT
GAGCTGGAGGAGATGGTGAAGGATCAGGAGACCACGGCCGAGCAGGCTCTGGAGGAGGAG
GCGCGGCGCCACCGCGAGGCCTACGGCAAGCTGGAGAGGGAGAAGGCTACCGAGGTGGAG
CTGCTCAATGCCAGGGTGCAGCAGTTGGAGGAAGAAAATACAGAGCTTAGAACAACAGTG
ACTCGGCTCAAGTCTCAAACAGAGAAACTGGATGAGGAGCGGCAGCGCATGTCTGACCGT
CTGGAGGACACCAGCCTGCGGCTCAAAGATGAGATGGACCTGTACAAGCGCATGATGGAC
AAGCTGCGACAGAACCGCCTTGAGTTCCAGAAGGAGCGGGAGGCGACGCAGGAGCTCATC
GAGGACTTGCGGAAGGAGCTGGAGCACCTGCAGATGTACAAGCTGGACTGCGAGCGGCCA
GGCAGGGGCCGCAGTGCCTCCTCTGGCCTAGGCGAGTTCAATGCCAGGGCCCGCGAGGTG
GAGCTCGAGCACGAGGTCAAGCGGCTCAAGCAGGAGAATTATAAGCTGCGGGATCAGAAC
GACGACTTGAATGGGCAGATTTTGAGCCTCAGCCTCTACGAAGCAAAAAACCTCTTTGCT
GCCCAGACTAAAGCCCAGTCTCTGGCTGCAGAGATAGACACCGCCTCGCGCGATGAGCTA
ATGGAAGCCCTGAAGGAGCAGGAGGAGATCAACTTCCGGCTGAGGCAGTACATGGACAAG
ATTATCCTCGCCATCCTGGACCACAATCCCTCCATCCTCGAGATCAAACAC

Protein sequence predicted based on nucleotide sequence

MAGGAGWSGAPAALLRSVRRLREVFEVCGRDPDGFLRVERVAALGLRFGQGEEVEKLVKY
LDPNDLGRINFKDFCRGVFAMKGCEELLKDVLSVESAGTLPCAPEIPDCVEQGSEVTGPT
FADGELIPREPGFFPEDEEEAMTLAPPEGPQELYTDSPMESTQSLEGSVGSPAEKDGGLG
GLFLPEDKSLVHTPSMTTSDLSTHSTTSLISNEEQFEDYGEGDDVDCAPSSPCPDDETRT
NVYSDLGSSVSSSAGQTPRKMRHVYNSELLDVYCSQCCKKINLLNDLEARLKNLKANSPN
RKISSTAFGRQLMHSSNFSSSNGSTEDLFRDSIDSCDNDITEKVSFLEKKVTELENDSLT
NGDLKSKLKQENTQLVHRVHELEEMVKDQETTAEQALEEEARRHREAYGKLEREKATEVE
LLNARVQQLEEENTELRTTVTRLKSQTEKLDEERQRMSDRLEDTSLRLKDEMDLYKRMMD
KLRQNRLEFQKEREATQELIEDLRKELEHLQMYKLDCERPGRGRSASSGLGEFNARAREV
ELEHEVKRLKQENYKLRDQNDDLNGQILSLSLYEAKNLFAAQTKAQSLAAEIDTASRDEL
MEALKEQEEINFRLRQYMDKIILAILDHNPSILEIKH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RAB11FIP4

Symbol Nomenclature :

RAB11FIP4

SYNONYM :

RAB11-FIP4

Designation :

FIP4-Rab11|arfophilin-2|rab11 family-interacting protein 4

Full Nomenclature :

RAB11 family interacting protein 4 (class II)

GENEID :

84440

HGNC :

30267

MIM :

611999

Vega :

OTTHUMG00000132787

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515989      ATGGCGGGCGGCGCGGGCTGGTCGGGCGCCCCCGCGGCTCTGCTGCGCTCCGTGCGCCGC
NM_032932.3       ATGGCGGGCGGCGCGGGCTGGTCGGGCGCCCCCGCGGCTCTGCTGCGCTCCGTGCGCCGC
                  ************************************************************

HsCD00515989      CTGCGCGAGGTGTTCGAGGTGTGCGGCCGCGACCCCGACGGCTTCCTGCGCGTGGAGCGC
NM_032932.3       CTGCGCGAGGTGTTCGAGGTGTGCGGCCGCGACCCCGACGGCTTCCTGCGCGTGGAGCGC
                  ************************************************************

HsCD00515989      GTCGCGGCGCTCGGACTGCGCTTCGGCCAGGGCGAGGAGGTGGAAAAACTTGTGAAATAT
NM_032932.3       GTCGCGGCGCTCGGACTGCGCTTCGGCCAGGGCGAGGAGGTGGAAAAACTTGTGAAATAT
                  ************************************************************

HsCD00515989      TTGGATCCCAACGACCTGGGGAGAATCAACTTCAAGGACTTTTGCCGGGGGGTGTTCGCC
NM_032932.3       TTGGATCCCAACGACCTGGGGAGAATCAACTTCAAGGACTTTTGCCGGGGGGTGTTCGCC
                  ************************************************************

HsCD00515989      ATGAAAGGGTGCGAGGAGCTGCTGAAGGATGTGCTGTCGGTGGAGAGCGCGGGGACGCTG
NM_032932.3       ATGAAAGGGTGCGAGGAGCTGCTGAAGGATGTGCTGTCGGTGGAGAGCGCGGGGACGCTG
                  ************************************************************

HsCD00515989      CCGTGCGCGCCAGAGATCCCAGACTGCGTGGAGCAGGGCAGCGAGGTCACAGGCCCCACC
NM_032932.3       CCGTGCGCGCCAGAGATCCCAGACTGCGTGGAGCAGGGCAGCGAGGTCACAGGCCCCACC
                  ************************************************************

HsCD00515989      TTTGCTGATGGCGAGCTCATCCCCAGGGAACCCGGCTTTTTTCCCGAGGACGAGGAGGAG
NM_032932.3       TTTGCTGATGGCGAGCTCATCCCCAGGGAACCCGGCTTTTTTCCCGAGGACGAGGAGGAG
                  ************************************************************

HsCD00515989      GCTATGACGCTGGCGCCACCTGAGGGCCCCCAGGAGTTGTACACAGACAGCCCCATGGAG
NM_032932.3       GCTATGACGCTGGCGCCACCTGAGGGCCCCCAGGAGTTGTACACAGACAGCCCCATGGAG
                  ************************************************************

HsCD00515989      AGCACTCAGAGCCTGGAGGGGTCTGTCGGGAGTCCTGCCGAGAAGGACGGGGGACTTGGG
NM_032932.3       AGCACTCAGAGCCTGGAGGGGTCTGTCGGGAGTCCTGCCGAGAAGGACGGGGGACTTGGG
                  ************************************************************

HsCD00515989      GGCCTGTTTCTGCCAGAAGACAAGTCCCTGGTCCACACTCCATCCATGACGACCTCAGAC
NM_032932.3       GGCCTGTTTCTGCCAGAAGACAAGTCCCTGGTCCACACTCCATCCATGACGACCTCAGAC
                  ************************************************************

HsCD00515989      CTTTCTACACACTCCACCACCTCGCTCATCAGCAATGAGGAGCAGTTTGAAGACTATGGG
NM_032932.3       CTTTCTACACACTCCACCACCTCGCTCATCAGCAATGAGGAGCAGTTTGAAGACTATGGG
                  ************************************************************

HsCD00515989      GAGGGTGACGATGTGGACTGTGCCCCCAGCAGCCCTTGCCCCGATGATGAGACCAGGACC
NM_032932.3       GAGGGTGACGATGTGGACTGTGCCCCCAGCAGCCCTTGCCCCGATGATGAGACCAGGACC
                  ************************************************************

HsCD00515989      AACGTCTACTCGGACCTGGGGTCTTCGGTGTCTTCCAGTGCGGGGCAGACGCCTAGGAAA
NM_032932.3       AACGTCTACTCGGACCTGGGGTCTTCGGTGTCTTCCAGTGCGGGGCAGACGCCTAGGAAA
                  ************************************************************

HsCD00515989      ATGCGGCACGTGTACAACAGCGAATTGCTAGATGTTTACTGCTCTCAATGCTGCAAGAAA
NM_032932.3       ATGCGGCACGTGTACAACAGCGAATTGCTAGATGTTTACTGCTCTCAATGCTGCAAGAAA
                  ************************************************************

HsCD00515989      ATCAACCTGCTCAATGACTTGGAAGCCCGACTGAAAAACCTGAAGGCCAACAGCCCCAAC
NM_032932.3       ATCAACCTGCTCAATGACTTGGAAGCCCGACTGAAAAACCTGAAGGCCAACAGCCCCAAC
                  ************************************************************

HsCD00515989      CGAAAGATCTCCAGCACGGCCTTTGGACGGCAGCTCATGCACAGCAGCAACTTCAGCAGC
NM_032932.3       CGAAAGATCTCCAGCACGGCCTTTGGACGGCAGCTCATGCACAGCAGCAACTTCAGCAGC
                  ************************************************************

HsCD00515989      AGCAATGGCAGCACCGAAGACCTGTTCCGGGACAGCATTGACTCTTGCGACAATGACATC
NM_032932.3       AGCAATGGCAGCACCGAAGACCTGTTCCGGGACAGCATTGACTCTTGCGACAATGACATC
                  ************************************************************

HsCD00515989      ACAGAGAAGGTAAGCTTCCTGGAAAAGAAGGTGACAGAGCTGGAGAATGACAGCCTGACC
NM_032932.3       ACAGAGAAGGTAAGCTTCCTGGAAAAGAAGGTGACAGAGCTGGAGAATGACAGCCTGACC
                  ************************************************************

HsCD00515989      AATGGGGACCTGAAGAGCAAGCTGAAGCAAGAGAACACACAGCTGGTGCACAGGGTGCAT
NM_032932.3       AATGGGGACCTGAAGAGCAAGCTGAAGCAAGAGAACACACAGCTGGTGCACAGGGTGCAT
                  ************************************************************

HsCD00515989      GAGCTGGAGGAGATGGTGAAGGATCAGGAGACCACGGCCGAGCAGGCTCTGGAGGAGGAG
NM_032932.3       GAGCTGGAGGAGATGGTGAAGGATCAGGAGACCACGGCCGAGCAGGCTCTGGAGGAGGAG
                  ************************************************************

HsCD00515989      GCGCGGCGCCACCGCGAGGCCTACGGCAAGCTGGAGAGGGAGAAGGCTACCGAGGTGGAG
NM_032932.3       GCGCGGCGCCACCGCGAGGCCTACGGCAAGCTGGAGAGGGAGAAGGCTACCGAGGTGGAG
                  ************************************************************

HsCD00515989      CTGCTCAATGCCAGGGTGCAGCAGTTGGAGGAAGAAAATACAGAGCTTAGAACAACAGTG
NM_032932.3       CTGCTCAATGCCAGGGTGCAGCAGTTGGAGGAAGAAAATACAGAGCTTAGAACAACAGTG
                  ************************************************************

HsCD00515989      ACTCGGCTCAAGTCTCAAACAGAGAAACTGGATGAGGAGCGGCAGCGCATGTCTGACCGT
NM_032932.3       ACTCGGCTCAAGTCTCAAACAGAGAAACTGGATGAGGAGCGGCAGCGCATGTCTGACCGT
                  ************************************************************

HsCD00515989      CTGGAGGACACCAGCCTGCGGCTCAAAGATGAGATGGACCTGTACAAGCGCATGATGGAC
NM_032932.3       CTGGAGGACACCAGCCTGCGGCTCAAAGATGAGATGGACCTGTACAAGCGCATGATGGAC
                  ************************************************************

HsCD00515989      AAGCTGCGACAGAACCGCCTTGAGTTCCAGAAGGAGCGGGAGGCGACGCAGGAGCTCATC
NM_032932.3       AAGCTGCGACAGAACCGCCTTGAGTTCCAGAAGGAGCGGGAGGCGACGCAGGAGCTCATC
                  ************************************************************

HsCD00515989      GAGGACTTGCGGAAGGAGCTGGAGCACCTGCAGATGTACAAGCTGGACTGCGAGCGGCCA
NM_032932.3       GAGGACTTGCGGAAGGAGCTGGAGCACCTGCAGATGTACAAGCTGGACTGCGAGCGGCCA
                  ************************************************************

HsCD00515989      GGCAGGGGCCGCAGTGCCTCCTCTGGCCTAGGCGAGTTCAATGCCAGGGCCCGCGAGGTG
NM_032932.3       GGCAGGGGCCGCAGTGCCTCCTCTGGCCTAGGCGAGTTCAATGCCAGGGCCCGCGAGGTG
                  ************************************************************

HsCD00515989      GAGCTCGAGCACGAGGTCAAGCGGCTCAAGCAGGAGAATTATAAGCTGCGGGATCAGAAC
NM_032932.3       GAGCTCGAGCACGAGGTCAAGCGGCTCAAGCAGGAGAATTATAAGCTGCGGGATCAGAAC
                  ************************************************************

HsCD00515989      GACGACTTGAATGGGCAGATTTTGAGCCTCAGCCTCTACGAAGCAAAAAACCTCTTTGCT
NM_032932.3       GACGACTTGAATGGGCAGATTTTGAGCCTCAGCCTCTACGAAGCAAAAAACCTCTTTGCT
                  ************************************************************

HsCD00515989      GCCCAGACTAAAGCCCAGTCTCTGGCTGCAGAGATAGACACCGCCTCGCGCGATGAGCTA
NM_032932.3       GCCCAGACTAAAGCCCAGTCTCTGGCTGCGGAGATAGACACCGCCTCGCGCGATGAGCTA
                  *****************************.******************************

HsCD00515989      ATGGAAGCCCTGAAGGAGCAGGAGGAGATCAACTTCCGGCTGAGGCAGTACATGGACAAG
NM_032932.3       ATGGAAGCCCTGAAGGAGCAGGAGGAGATCAACTTCCGGCTGAGGCAGTACATGGACAAG
                  ************************************************************

HsCD00515989      ATTATCCTCGCCATCCTGGACCACAATCCCTCCATCCTCGAGATCAAACAC---
NM_032932.3       ATTATCCTCGCCATCCTGGACCACAATCCCTCCATCCTCGAGATCAAACACTAA
                  ***************************************************