Gene:
Gene Symbol:
ECU10_0750
Gene Name:
METHIONINE AMINOPEPTIDASE 2
Original Clone ID:
9201a1KWt4
Keyword:
None
Species:
Encephalitozoon cuniculi
Type:
cDNA
Vector Name:
pFASTBAC1-C-His-TOPO
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
EcCD00531165
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1107nts
Open reading frame: 51 to 1157
GTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTA
AGTGTATTCTGTTGAATCAGGCTGAGGAGCTTCCCATCGAGTTCCTCCCAAAAGATGGGG
TTTACGGGAAGGGAAAGCTGTTCGATTCAAGGAACATGGAGATTGAGAACTTCACAGAGT
CTGACATTCTGCAGGATGCACGGAGGGCGGCGGAGGCTCACAGAAGAGCACGCTACAGGG
TTCAGAGCATCGTAAGGCCGGGGATAACGCTTCTTGAGATTGTAAGGTCCATAGAGGACT
CCACCAGGACGCTTCTGAAAGGTGAAAGAAACAACGGAATAGGATTTCCTGCAGGGATGT
CGATGAACTCCTGTGCAGCACACTACACAGTCAACCCTGGGGAGCAGGACATTGTCCTCA
AGGAAGATGATGTCTTGAAGATAGACTTCGGGACACACTCCGACGGCAGGATAATGGACT
CTGCCTTCACAGTTGCATTCAAGGAAAATCTTGAGCCTTTGCTGGTTGCTGCCAGGGAGG
GGACGGAGACAGGTATCAAGTCACTAGGGGTGGATGTGAGAGTCTGCGACATAGGGAGAG
ACATCAACGAAGTCATTTCGAGCTATGAGGTGGAGATTGGTGGGCGGATGTGGCCAATCA
GACCGATATCGGATCTCCATGGTCACTCCATATCCCAGTTCAGGATCCATGGAGGAATCT
CGATACCGGCTGTGAACAACAGGGACACAACAAGGATCAAGGGAGACTCGTTCTATGCAG
TGGAAACGTTTGCAACGACCGGAAAAGGATCCATAGACGACAGGCCCCCATGCTCGCACT
TCGTGCTGAACACATACAAGAGCAGGAAGCTCTTCAACAAGGACCTAATAAAGGTCTACG
AATTTGTCAAGGACTCGCTTGGAACTCTCCCCTTCAGTCCCAGACATCTGGACTACTACG
GCCTGGTGAAGGGCGGATCCCTGAAGAGCGTGAACTTGCTCACAATGATGGGGCTCCTCA
CCCCCTATCCTCCCCTTAACGACATCGACGGATGCAAGGTTGCACAGTTCGAGCACACAG
TATATTTAAGTGAACACGGGAAGGAGGTCCTTACAAGGGGCGATGACTATGAAGGGCATC
ATCACCATCACCACTGA
Protein sequence predicted based on nucleotide sequence
MALKCILLNQAEELPIEFLPKDGVYGKGKLFDSRNMEIENFTESDILQDARRAAEAHRRA RYRVQSIVRPGITLLEIVRSIEDSTRTLLKGERNNGIGFPAGMSMNSCAAHYTVNPGEQD IVLKEDDVLKIDFGTHSDGRIMDSAFTVAFKENLEPLLVAAREGTETGIKSLGVDVRVCD IGRDINEVISSYEVEIGGRMWPIRPISDLHGHSISQFRIHGGISIPAVNNRDTTRIKGDS FYAVETFATTGKGSIDDRPPCSHFVLNTYKSRKLFNKDLIKVYEFVKDSLGTLPFSPRHL DYYGLVKGGSLKSVNLLTMMGLLTPYPPLNDIDGCKVAQFEHTVYLSEHGKEVLTRGDDY EGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Tested
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
Find experimental protocols in NYSGXRC-9201a
Recommended expression in:
Not Applicable
Vector Name:
pFASTBAC1-C-His-TOPO
Synonyms:
pFB1-C-His-TOPO, pFB1-C-His-TOPO_KW
Sequencing Primer:
Forward:
pFB forward
Reverse:
pFB reverse
Description:
Baculovirus vector, adds C-terminal 6xHis tag and TEV protease site; amp resistance; TOPO cloning.
Comments:
None
Size (bp):
4763
Parent Vector:
None
Properties:
TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation ATG (Met, Ala, Leu)
4065
4078
TOPO recognition site
TOPO
TOPO cloning site
4069
4078
bacterial origin
ori
pUC-type bacterial origin of replication
1544
2226
poly-A signal
polyA
SV40 polyA signal sequence
4189
4380
primer
pFB foward
forward sequencing primer: TATTCCGGATTATTCATACC
3995
4014
primer
pFB reverse
reverse sequencing primer: ACAAATGTGGTATGGCTGATT
4150
4170
promoter
PH
polyhedrin promoter for insect cell expression
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1 ori
f1 origin
19
325
tag
His
C-terminal 6xHis and stop
4079
4096
transposon fragment
Tn7L
Tn7L transposon site
1466
12
transposon fragment
Tn7R
Tn7R transposon site
2709
2933
