Gene:
Gene Symbol:
EBI_27249
Gene Name:
methionine aminopeptidase
Original Clone ID:
9201b2KFt1
Keyword:
None
Species:
Enterocytozoon bieneusi
Type:
cDNA
Vector Name:
pSGX-TOPO
Format:
FUSION
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
EbCD00532104
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1011nts
Open reading frame: 51 to 1061
ACGATTACGATCTACCAACGACCGAAAACCTGTATTTTCAGGGATCCCTTATGATTCTAA
TTAATCCTGTTCAAGAAAAACCTATTGAATTTCTTGATAAGACTTTATTTAATACAACTA
GTGATACGCAATTAAATGATATTATGCGAGCTGCTGAAGCACATAAGCGCATTCGTGGAA
ATTTACAAAAAATACTTAAACCAGGATGTTCAGTAAGAGAAATTATAAAATATGTTGAAG
ATTCTACACGAATAATGTTGCATGGTGAAAAAAATAATGGAATCGGTTTTCCATGTGGGG
TAAGCTTAAACAATGTAGCGGCACATTTTAGTCTTAATCCTTGGAATGAAGATATTATTT
TAACTAAAAATGATGTATTAAAAATTGATTTTGGAACTCATTCAAATGGTAGAATTGTTG
ATTCTGCGTTTAGTGTTTCTTTTGATGAAAAATATACAAATTTGTTATTAGCCACGAAAG
AAGCTACTGAAAGAGGAATAAAAGTGATTGGAATAGATATGGCTGTTTGTGAAATTGGAA
AGGAAATTTCTGAAGTATTTAAATCATTTGAATTTGAAGAAAATGGAGAAATGATAAATA
TTAAACCCATATGGAATTTAAATGGCCATTCAATAGATCAATATAAAATACATGCTGGAA
TATCTATTCCATCTATTAATAATGGTGATTATTCACGTATAACACCAGGATTTTTTGCTA
TAGAGACATTTGCTAGTACAGGAATAGGTGAAACTAAGGATTATGGAGAATGTTCTCATT
TTGCTTTAGCTTCTAGACCAACTACAAATAAAATATATGATAAAAAAAATGAAGAAATTT
TGAATTTAATTAAAAAAGAATTTGGAACACTTCCATTTAGTCATCAACATTTGCTATATT
ATACTCAAAATGCTAAAGCAGCTGTGCAGTTATTGGCTGCTAGAAAATTTATAGATTCAT
ATCCTCCATTAGTTGATATTCCAAAGAGTTATGTGGCACAATTTGAACACACACTGTATA
TTACTGAAAAAAGCAAATTTAATATATCCAAAGGTGACGAT
Protein sequence predicted based on nucleotide sequence
MILINPVQEKPIEFLDKTLFNTTSDTQLNDIMRAAEAHKRIRGNLQKILKPGCSVREIIK YVEDSTRIMLHGEKNNGIGFPCGVSLNNVAAHFSLNPWNEDIILTKNDVLKIDFGTHSNG RIVDSAFSVSFDEKYTNLLLATKEATERGIKVIGIDMAVCEIGKEISEVFKSFEFEENGE MINIKPIWNLNGHSIDQYKIHAGISIPSINNGDYSRITPGFFAIETFASTGIGETKDYGE CSHFALASRPTTNKIYDKKNEEILNLIKKEFGTLPFSHQHLLYYTQNAKAAVQLLAARKF IDSYPPLVDIPKSYVAQFEHTLYITEKSKFNISKGDD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
K180E; I235T
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: insect cells Marker: gentamicin
Host Type: insect cells Marker: gentamicin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Tested
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
Find experimental protocols in NYSGXRC-9201b
Recommended expression in:
Not Applicable
Vector Name:
pSGX-TOPO
Synonyms:
pSGX-TOPO_KF
Sequencing Primer:
Forward:
pFB forward
Reverse:
pFB reverse
Description:
Baculovirus vector, adds N-terminal 6xHis tag and TEV protease site; ampicillin resistance in bacteria (gentamicin resistance in insect cells); TOPO cloning.
Comments:
pFastBAC topogated N-His with TEV.
Size (bp):
4793
Parent Vector:
None
Properties:
TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation ATG
4050
4052
TOPO recognition site
TOPO
TOPO cloning site
4123
4132
bacterial origin
ori
pUC-type origin of replication
1544
2226
poly-A signal
polyA
SV40 poly-A signal sequence
4222
4413
primer
pFB foward
forward sequencing primer: TATTCCGGATTATTCATACC
3995
4014
primer
pFB reverse
reverse sequencing primer: ACAAATGTGGTATGGCTGATT
4183
4203
promoter
Pc
Pc promoter
0
0
promoter
PH
polyhedrin promoter
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1 ori
f1 origin
19
325
tag
His
6xHis tag
4062
4079
transposon fragment
Tn7L
Tn7L transposon sequence
0
0
transposon fragment
Tn7R
Tn7R transposon sequence
2709
2933
