DNASU Plasmid | MED25 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: MED25

Gene Name: mediator complex subunit 25

Original Clone ID:

HR6188A-397-543-14.39

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI
Northeast Structural Genomics Consortium

HsCD00539749

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 447nts

Open reading frame: 51 to 497

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGAATAAGC
TTCTGGCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATG
CCAACACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACC
TGAAGACGGAGCAGTGGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGA
CCATCCTGGGCCCTTTGTTCCGGAACTCAAGGATGGTCCAGTTCCATTTCACCAACAAGG
ACCTGGAGTCTCTCAAAGGCCTCTACCGCATCATGGGCAACGGCTTCGCGGGCTGCGTGC
ACTTCCCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCCTGTACTCGTCCAAGA
AGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTTGTCAACGGCATCCGGC
AGGTCATCACCAACTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTT
GGCTGCTG

Protein sequence predicted based on nucleotide sequence

MNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQ
QLLTILGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLY
SSKKKIFMGLIPYDQSGFVNGIRQVITN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c194t,T65I;c414t,F138F

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR6188A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

MED25

Symbol Nomenclature :

MED25

SYNONYM :

ACID1; ARC92; CMT2B2; P78; PTOV2

Designation :

ARC/mediator transcriptional coactivator subunit|activator interaction domain-containing protein 1|activator-recruited cofactor 92 kDa component|mediator of RNA polymerase II transcription subunit 25|mediator of RNA polymerase II transcription, subunit 25 homolog|prostate-derived protein 78 kDa

Full Nomenclature :

mediator complex subunit 25

GENEID :

81857

Locus Tag :

TCBAP0758

GI :

50428940

GenBank Accession :

NM_030973

HGNC :

28845

MIM :

610197

Vega :

OTTHUMG00000183161

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00539749      ------------------------------------------------------------
NM_030973.3       ATGGTCCCCGGGTCCGAGGGCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTT
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GTGATTGAGGGTACGGCCAACCTGGGACCCTACTTCGAGGGGCTCCGCAAGCACTACCTG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CTCCCGGCCATCGAGTATTTTAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGAC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       TATGGGGGGACCCAGTACAGCCTCGTGGTGTTCAACACAGTGGACTGCGCTCCCGAGTCC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       TACGTACAATGTCACGCTCCCACCAGCAGCGCCTATGAGTTTGTCACCTGGCTCGATGGC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       ATTAAGTTCATGGGCGGGGGTGGTGAGAGCTGCAGCCTCATCGCGGAAGGACTCAGCACA
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GCCTTGCAGCTGTTTGATGACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GTCTGCCTCCTCATCTGCAACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       TACTCTGGATGCACAACTGAGAATCTTGTGCAGCAGATTGGGGAGCGGGGGATCCACTTC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       TCCATTGTGTCTCCCCGGAAGCTGCCTGCGCTTCGGCTTCTGTTTGAGAAGGCAGCCCCC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CCGGCCTTGCTGGAGCCGCTGCAGCCTCCGACAGATGTGAGCCAGGACCCGAGGCACATG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GTGCTGGTTCGGGGACTCGTGCTGCCTGTTGGGGGTGGCTCAGCCCCAGGCCCCCTCCAG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       TCAAAGCAGCCAGTCCCCCTGCCTCCCGCCGCACCCTCAGGTGCCACTCTCTCAGCAGCC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CCCCAGCAGCCTCTGCCCCCCGTCCCCCCGCAGTACCAGGTTCCCGGGAACCTGAGTGCA
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GCTCAGGTGGCCGCGCAGAATGCAGTGGAGGCTGCCAAGAACCAGAAGGCTGGGCTGGGC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CCTCGCTTCTCGCCCATCACCCCTCTCCAACAAGCTGCTCCCGGAGTGGGTCCCCCCTTC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       AGCCAGGCCCCAGCTCCCCAACTACCCCCAGGACCCCCTGGCGCCCCCAAGCCACCACCT
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GCTTCCCAGCCCAGTCTGGTCTCCACTGTGGCCCCTGGCTCCGGCCTGGCTCCCACGGCA
                                                                              

HsCD00539749      ------------------CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATC
NM_030973.3       CAGCCCGGGGCACCGTCCATGGCAGGCACTGTGGCCCCAGGAGGGGTGAGCGGCCCTTCC
                                    .*  .**... :*: . .*** *.*  .* * *. *.* : *

HsCD00539749      ACAGCCATATG-------------------------------------AATAAGCTTCTG
NM_030973.3       CCAGCCCAGCTGGGAGCCCCAGCCCTCGGTGGGCAGCAGTCAGTCTCCAATAAGCTTCTG
                  .*****.:.                                       ************

HsCD00539749      GCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATGCCAAC
NM_030973.3       GCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATGCCAAC
                  ************************************************************

HsCD00539749      ACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACCTGAAG
NM_030973.3       ACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACCTGAAG
                  ************************************************************

HsCD00539749      ACGGAGCAGTGGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGACCATC
NM_030973.3       ACGGAGCAGTGGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGACCACC
                  ********************************************************** *

HsCD00539749      CTGGGCCCTTTGTTCCGGAACTCAAGGATGGTCCAGTTCCATTTCACCAACAAGGACCTG
NM_030973.3       CTGGGCCCTTTGTTCCGGAACTCAAGGATGGTCCAGTTCCATTTCACCAACAAGGACCTG
                  ************************************************************

HsCD00539749      GAGTCTCTCAAAGGCCTCTACCGCATCATGGGCAACGGCTTCGCGGGCTGCGTGCACTTC
NM_030973.3       GAGTCTCTCAAAGGCCTCTACCGCATCATGGGCAACGGCTTCGCGGGCTGCGTGCACTTC
                  ************************************************************

HsCD00539749      CCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCCTGTACTCGTCCAAGAAGAAG
NM_030973.3       CCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCCTGTACTCGTCCAAGAAGAAG
                  ************************************************************

HsCD00539749      ATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTTGTCAACGGCATCCGGCAGGTC
NM_030973.3       ATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAACGGCATCCGGCAGGTC
                  ************************************** *********************

HsCD00539749      ATCACCAACTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
NM_030973.3       ATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAGCAGCAGCGAGGAATGGGG
                  ********* * .:  **.***.**:**:.*...:* .  *...*.*** ..*:: *  *

HsCD00539749      CTG---------------------------------------------------------
NM_030973.3       GGACAGCAGGCACCCCCAGGGCTGGGGCCCATTCTGGAGGACCAAGCCAGGCCCTCACAG
                    .                                                         

HsCD00539749      ------------------------------------------------------------
NM_030973.3       AATCTGCTCCAGCTCCGCCCACCGCAGCCCCAGCCTCAGGGTACCGTAGGGGCCTCTGGG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GCCACGGGGCAGCCCCAGCCCCAAGGTACTGCCCAGCCCCCGCCAGGTGCCCCTCAAGGC
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CCTCCTGGAGCAGCTTCTGGCCCACCCCCTCCTGGACCCATCCTTCGGCCCCAGAACCCT
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GGGGCCAACCCTCAGCTGCGAAGCCTCCTCCTCAACCCACCACCGCCGCAGACTGGGGTG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CCCCCACCCCAGGCCTCCCTCCACCACCTCCAGCCACCAGGGGCTCCTGCGCTGCTGCCT
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       CCGCCGCACCAGGGCCTGGGGCAGCCCCAGTTGGGGCCCCCACTCCTGCATCCACCACCT
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       GCCCAGTCCTGGCCCGCACAACTTCCCCCTCGGGCTCCACTGCCAGGTCAGATGCTGCTG
                                                                              

HsCD00539749      ------------------------------------------------------------
NM_030973.3       AGCGGGGGTCCCCGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGAC
                                                                              

HsCD00539749      ------------------------
NM_030973.3       GACATCCTCATGGATCTCATCTGA

Pathways

Reactome :

Developmental Biology

Reactome :

Fatty acid, triacylglycerol, and ketone body metabolism

Reactome :

Gene Expression

Reactome :

Generic Transcription Pathway

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

PPARA activates gene expression

Reactome :

Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Reactome :

Transcriptional regulation of white adipocyte differentiation

Protein

PDB - Structural Annotation :

2L6U

Structural Annotation Wiki :

HR6188A

UniProt :

Q71SY5

HPRD :

14380

Protein Structure Viewer

2D Image
3D Image (Jmol)

FirstGlance in Jmol

PDB ID

Style

Color

Clone

Cloning Information :

620057

HIP Master Clone ID :

517038

Original Clone ID :

HR6188A-397-543-14.39

Experimental

Structural Biology Knowledgebase :

SBKB (2L6U)

TargetTrack - Experimental data :

HR6188A

Organism

Species Specific ID:

81857

Genome

Chromosome :

Map Location :

19q13.3

Ensembl :

ENSG00000104973

Reagents

Labome :

MED25-antibody

MED25 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

MED25 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)