PF1855 (P. furiosus )
In pET15TEV_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PF1855
Gene Name:  transcription-associated protein TFIIS
Original Clone ID:
PfR225-1-424-TEV.88
Keyword:
full length protein truncation
Species:
Pyrococcus furiosus
Type:
cDNA
Vector Name:
pET15TEV_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Northeast Structural Genomics Consortium
PfCD00586111
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AVAILABLE
Academic and non-profit labs
Special MTA: PSI/NESG
Insert sequence: 1275nts
Open reading frame: 7 to 1281

AGCCATATGCTCATCCACATAGGTATTGACGACACCGATTCACCAAATGGAATGTGCACT
ACATATATTGGGGCTATTCTATATAGGGAAATTTCCAAGATAGCTGAGCCTCTAGACTTT
CCAAGATTAATTAGGCTAAATCCAAATGTTCCTTACAAGACCAGGGGAAATGGGGCCGTT
GCAATGAGTTTTAAAATCGATGAAGAAAAGATAAAGGAAGTAAAGACACTTGTAATACGG
TATGTAAGGGAATTGGCCGATATAGATCACGAGAACACAAATCCAGGGATTGTTTTCTTA
ATTGGGGAAGTGCCAAAGGAATTAGAAGAGTTTTCTCTAAGAGCATTAAGAGAGCACGTT
ACAATCGAAGAAGCAGAACATGTAGCTCGAAAAGTCAACGCAGAAGTTTACAAATTCAAG
CTCGGAAGAGGAATAATTGGGGGATTAGCAGCTATAGGCTATCCTCTAGAAAAGTTCACT
TACGAATTACTTGCCTACAGGAAAAGAGAGTACTGGGGAACTCCAAGAAGAGTTATTAAG
GAAAGTGTATTTTATGCAGACAAATGGAGCTATCCGTTCACCTACGATAACGTTGACCCT
TACAAGAGAACTGTCCTAATAACTCCTCATGGAAAAGACCCAGTTCTTGTGGGGATAAGA
GGAATTGATGTAGGAAAGATTCTTCAGGTCTTTGAGATGATTAAAATAGAAGAACCAATT
GAATTCTTCCAAGTTTACAAAACAAATCAAAATACCGACGATCATTTGGTTGTTAAGAAG
ATAAAAGATGTTAAGCCTTATGATAACGTTGTCATAAGGGGAAAAGTCGCTGGAAAATAC
TGGGAAAAAGGAAGACATGTATTTTTTGAACTTGAGGATGAAACAGGGAAGATTAGAGTG
GCAGCTTTTGAACCAACTAAAAAGTTTAGAAACTACGTGAGGAAACTCCTCCCAGGAGAT
GAGGTTATAATAGCTGGCGGAGTCAAAGAATTCGAAGGAGTATTAACCGTAAACCTGGAA
AAATTTTACCCAGTAAATCTTGTTCCCAAAGTGGAATATGAAAAGCCAAGATGTCCAAAG
TGCGGAGGAACGATGAAGAGCAAGGGAGATTATCTAAAGTGTAAAAAGTGCGGATATAAG
ATGGAAAAAATATTGACTCCAGTTAAGAGGCCAAGAGACTTAAAGAAGAAGATTTATGAA
GTTCCTCCAGATGCTCGGAAACATATTTCTCGCCCCTTAGTTCTCCCAGGAGGGGAAGAT
AAGATATTAGAGGCTCTATAG

Protein sequence predicted based on nucleotide sequence

MLIHIGIDDTDSPNGMCTTYIGAILYREISKIAEPLDFPRLIRLNPNVPYKTRGNGAVAM
SFKIDEEKIKEVKTLVIRYVRELADIDHENTNPGIVFLIGEVPKELEEFSLRALREHVTI
EEAEHVARKVNAEVYKFKLGRGIIGGLAAIGYPLEKFTYELLAYRKREYWGTPRRVIKES
VFYADKWSYPFTYDNVDPYKRTVLITPHGKDPVLVGIRGIDVGKILQVFEMIKIEEPIEF
FQVYKTNQNTDDHLVVKKIKDVKPYDNVVIRGKVAGKYWEKGRHVFFELEDETGKIRVAA
FEPTKKFRNYVRKLLPGDEVIIAGGVKEFEGVLTVNLEKFYPVNLVPKVEYEKPRCPKCG
GTMKSKGDYLKCKKCGYKMEKILTPVKRPRDLKKKIYEVPPDARKHISRPLVLPGGEDKI
LEAL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in PfR225
Recommended expression in:
Not Applicable