Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
SR577-14.1
Keyword:
targeted domain
Species:
Bacillus subtilis
Type:
cDNA
Vector Name:
pET15_NESG
Format:
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
Northeast Structural Genomics Consortium
BsCD00627755
Price:
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Special MTA: PSI/NESG
Insert sequence: 879nts
Open reading frame: 1 to 879
ATGGGGAATAACTCAGAAAGCAAAGGTTCTGCCTCTGATTCAAAAGGCGCAGAAACCTTT
ACATACAAGGCTGAAAACGGAAATGTAAAAATCCCGAAGCATCCTAAACGGGTGGTTGTC
ATGGCAGACGGCTATTACGGCTACTTCAAAACACTCGGCATCAACGTTGTAGGTGCGCCT
GAGAATGTATTTAAAAACCCTTACTACAAAGGAAAAACCAACGGCGTCGAAAACATCGGA
GACGGCACTTCGGTTGAAAAGGTCATTGATTTGAACCCTGACCTGATTATCGTCTGGACA
ACACAGGGCGCTGATATTAAAAAGCTTGAAAAAATTGCGCCGACTGTCGCAGTAAAATAC
GATAAGCTCGACAACATCGAACAGCTGAAAGAATTTGCAAAAATGACGGGAACTGAAGAT
AAAGCTGAAAAATGGCTGGCCAAGTGGGATAAAAAAGTTGCGGCCGCTAAAACAAAAATC
AAAAAAGCGGTCGGTGACAAAACCATTTCCATTATGCAAACGAACGGAAAAGACATTTAC
GTATTTGGTAAAGACTTTGGAAGAGGCGGAAGCATCATTTACAAAGACCTCGGCCTGCAG
GCTACAAAGCTGACAAAAGAAAAAGCCATTGACCAGGGGCCAGGTTATACAAGTATTTCA
TTAGAGAAGCTCCCTGACTTCGCCGGAGATTACATTTTCGCGGGCCCATGGCAATCAGGC
GGTGATGATGGCGGCGTTTTTGAAAGCTCCATTTGGAAGAACCTGAATGCCGTTAAAAAT
GGCCATGTATACAAGATGGATCCGATCGGATTCTATTTCACAGACCCGATTTCATTAGAA
GGACAACTGGAGTTTATTACGGAAAGCTTGACTAAATAG
Protein sequence predicted based on nucleotide sequence
MGNNSESKGSASDSKGAETFTYKAENGNVKIPKHPKRVVVMADGYYGYFKTLGINVVGAP ENVFKNPYYKGKTNGVENIGDGTSVEKVIDLNPDLIIVWTTQGADIKKLEKIAPTVAVKY DKLDNIEQLKEFAKMTGTEDKAEKWLAKWDKKVAAAKTKIKKAVGDKTISIMQTNGKDIY VFGKDFGRGGSIIYKDLGLQATKLTKEKAIDQGPGYTSISLEKLPDFAGDYIFAGPWQSG GDDGGVFESSIWKNLNAVKNGHVYKMDPIGFYFTDPISLEGQLEFITESLTK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in SR577
Recommended expression in:
Not Applicable
Vector Name:
pET15_NESG
Synonyms:
pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments:
Modified Novagen pET vector-common MCS
Size (bp):
5723
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium
Jessica Bearden
Northeast Structural Genomics Consortium
Publications:
PMID:
15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)
327
452
bacterial origin
ori
ColE1 origin of replication
3881
4563
gene fragment
ROP
repressor of primer ROP sequence
3281
3457
primer
T7 forward
T7 forward sequencing primer
481
496
primer
T7 reverse
T7 reverse sequencing primer
257
275
promoter
T7
T7 promoter
478
497
repressor binding site
lacO
lacO regulatory sequence
456
478
selectable marker
AmpR
ampicillin resistance gene
4661
5230
tag
His
N-terminal 6x His tag
387
404
trxn termination sequence
T7 term
T7 termination sequence
257
275
