Center for Membrane Proteins of Infectious Diseases
Description:
Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI
Comments:
None
Publications:
PMID:26908053 Title: Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization
Authors:
Arizona State University
Medical University of South Carolina
Debra T. Hansen
Jeffrey L. Hansen
Thirumagal Thiyagarajan
Center for Membrane Proteins in Infectious Diseases
Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI
Comments:
For arabinose-inducible expression of the insert, on a vector that is compatible with most other cloning vectors (non-ColE1 origin of replication; Clone by ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI
Size (bp):
3552
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Arizona State University
Medical University of South Carolina
Debra T. Hansen
Jeffrey L. Hansen
Thirumagal Thiyagarajan
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
CloDF13
CloDF13 origin
2792
3530
primer
forward primer
ARAC5R forward sequencing primer GGGATCATTTTGCGCTTCAG