Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH230476.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pDEST17
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
FtCD00063145
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4077nts
Open reading frame: 1 to 4077
ATGTCTTACTCATACGCTGAGAAAAAAAGAATTCGTAAAGAGTTTGGGGTTCTTCCTCAT
ATCTTAGATGTACCATATTTGCTTTCTATTCAAACAGAGTCATACAAAAAATTCTTGACT
GCAGATGCAGCAAAAGGAAGACTTCACTCTGGACTTGAAATAGTTTTAAAACAGTCTTTT
CCTGTTGAGAGTAAAAATGGTCAATATGAACTTCACTATGTTGATTATCAAATTGGTGAG
CCTACTTTTGATGAAACTGAATGTCAGGTTCGTGGTGCTACTTATGATGCACCATTAAAT
GTGAAGTTAAGATTAGTTGTATATAATAAAGATGCTCTTCCAAATGAGAAAATTGTCGAG
GACATTAGAGAAGAGTATGTGTACATGGGCGATATTCCGCTAATGACAACTAATGGTACT
TTTATCATTAATGGGACAGAAAGAGTAGTAGTCTCTCAGTTGCATAGATCACCAGGGGTG
TTCTTTAGTAAGGATGACTCTGAGGAAGGAGCATTCTCAGCACGTATTATCCCATATAGA
GGCTCATGGCTTGATTTTGAATTTGACTCAAAAGGTATTATATGGGCTAGGATTGATAGA
AAAAGAAAATTCTGTGCTACAGTTATTTTAAAAGCTCTTGGTTATACTCAAGAGCAAATA
TTAGAGAATTTTTCTGAGAGTAAGACTATTACTTTTAATAGTAAAGGTTTTGCTCTTAGA
CTTGATAACCTTTCAAATATGAAAGGTGAGCTGCTTAAGTTTGACATTGTAGATGCTCAA
GATAATGTTATTGTTAAAAAGAATAAAAAATTAACTTCAAGAGATGTTAAGAAAATTAAA
GATGCAGGCGTTGACTCTGTAGCTATTGATTTTGATTTGGTCAGCACTCTTAGAGTTGCA
AAAGATATCGTTAATGAAGCAACAGGTGAAGTGATAGCTTATGCTAATGACGATGTTACA
GAAAGTTTATTAGAGTCATGCGTAGAAGTTGGCATGCTTGAATTAGAAGTTATTGATTTT
ATAACTACTGAAAGAGGTAGGTATATATCTGATACTTTAAAATATGATCTTACAAGAAAT
ACTGATGAGGCACTTGTCGAGATATATAAGGTATTACGACCAGGTGATCCTCCTGCAGCA
GCTTCAGTTAAAGCATTATTTGAAGGTTTATTCTTTATTGAAAGTAGATATAGCCTTTCT
GATATTGGTAGAATGAAGCTAAATGCTAGATTAGGTTCGGATAAGGTATCTAAAGATATC
TATACTTTAGAAAATAGTGATATAGTTGGCGTAATTGAAGAGCTTATAAATATCCGTGAT
GGTAAAGGTAAAGTTGATGATATTGATCATTTAGGTAACAGACGTGTACGTTCTGTTGGC
GAGATGGTTGAGAATCAGTTTAGAATAGGCCTCTACCGTGTAGAGAAAGGTATTCGTGAA
AGTATGTCTTTAGTACATAAAGATAAGCTTATGCCAAAAGATATCGTTAACTCGAAGCCA
ATCACAGCAGCAATTAAAGAATTCTTTACTTCTGGAGCGTTATCACAATTTATGGATCAA
GATAACCCATTGTCAGAAGTTACACATAAGCGTAGAATATCTGCATTAGGTCCAGGTGGT
TTATCACGTGATAGAGCAGGTTTTGAAGTACGTGATGTTCATGCAACACACTATGGTAGA
TTATGTCCAATTGAGACTCCAGAAGGTCCAAATATTGGTCTAATTAACTCATTAGCAAGT
TATGCTCGTGTTAATGATTATGGTTTCTTAGAAGCACCATATCGAAAAGTTGTTGATGGT
AAGGTTACAGATGAAATTGAATACTTATCTGCTATCGATGAAGATAATTATGTAATTGCT
CAAGCATCAACTAAACTTGATGAAAATAACCATTTTGTTGAAGATCTTATTCAATGTCGT
TCTGGTGGTGAGGCAATATTTACTGAGTCAAGTAGAGTTCAGTATATGGATGTTTCTGCT
AAACAGATGGTTTCAGCAGCTGCGGCACTAATTCCTTTCCTTGAGCATGATGATGCTAAC
AGGGTATTGATGGGTGCAAACATGCAACGTCAAGCGGTACCGACTCTTAAATCTGAGAAG
CCTTTAGTCGGTACAGGTATGGAGAAGATTGTTGCTAGAGATTCTGGTAATTGTATTATC
GCAAGAAATGCTGGTGAAGTGGCTGAAGTTGATTCTAACAGAATCGTTATTAAAGTAGAT
ACTGAAAAATCACAAACTAGTAATTTGGTTGATATTTATAGTTTAACTAAATTTAAACGC
TCAAACAAAAATACTTGTATTAACCAGCGTCCAATAGTAAATGTGGGTGATAAAGTAGAA
GCTGGTGATATTTTAGCGGATGGTTTTGCAACTGATTTTGGTGAATTGTCACTAGGGCAT
AACTTGATGGTTGCTTTTATGCCATGGAATGGTTATAACTTTGAGGATTCGATCTTACTA
TCTGAAAGAATAGTAAAAGATGATAAGTATACTAGTATCCATATTGAAGAGTTCACATGT
GTGGCTCGTGATACTAAGCTTGGTCCTGAAGAGATAACTGCAGATATTCCAAATGTTAGT
GAGTCTAGTTTGGCAAAACTTGATGAATCAGGTATCGTCCATATTGGCGCTAATGTCGAA
GCAGGCGATATTTTGGTAGCTAAGATTACTCCAAAAGCTGAGCAACAACTAACTCCTGAA
GAGAGACTACTTAGAGCAATCTTTAATGAAAAAGCATCAAATGTTGCTGATAGTTCATTA
AGAATGCCAAGTGGTACTTCTGGTACGGTTATTAATGTTCAAGTATTTGAGAATGACAAA
GGTGGTAAAAGTAAGCGAGCTCTTAAGATTGAAAAAGAACTGATCGATAAAGCTCGTAAG
GATTTTGATGAGGAGTTTGCAGTAATTGAATCTGTAGTTAAATCATCAATTGAGCAAGAA
GTTGTTGGTGCAAAAATCCAAAAAGCAAAAGGTCTTAAAAAAGGTGCTATCCTTACAAAA
GAATTTTTAGCAACACTACCTTTCTCTAAATGGCTAGAAATTTCTTTTGAGGATGAGAAA
CTTGAAGAGAAAGTTCAAAACGCTAGGGAGTATTATGAGGAAGCTAAAATTGCTATAGAT
GCTAAATTTGAAGCTAAGAAAAAATCTATTACTCAGAGTAATGAGCTATCTCCAGGTGTG
CTTAAGACTGTTAAAGTTTTTGTTGCGATTAAAAAGCGTATCCAGCCAGGTGATAAGATG
GCTGGTCGTCATGGTAACAAAGGTGTGGTTTCACGCGTATTACCAGTTGAAGATATGCCA
TATATGGAAGATGGTACTCCTGTAGACGTTTGTCTAAATCCTTTAGGTATCCCATCACGT
ATGAACATTGGGCAGATTTTAGAAGCACACTTAGGATTGGCTTCATATGGTTTAGGTAAA
AAAATCGAAAAAACTTTAGAGAAAACTAGAAAAGCAGCTGAACTAAGAAAGACTTTAGAA
GAAGTATATAATAGCGTTGGCGATAAGAAAGTTAATCTTGAGGCTTTAAATGACGAAGAG
ATACTAACTCTTTGTGACAACCTAAAAGGTGGAGTACCAATCGCTACACCAGTATTTGAT
GGTGCTAAAGAAGAAGATATTAAATCACTACTGAAAATAGGCGGCTTTGCAACTAATGGT
CAGATGAAGCTTTTTGATGGTCGTACTGGTAAGCCTTTTGATAGACATGTAACTGTTGGT
TATATGTATATGCTAAAACTAGATCACTTAGTAGATGATAAGATGCATGCAAGATCGACT
GGCTCATATAGTCTAGTTACGCAGCAACCATTAGGTGGTAAAGCACAGTTTGGTGGACAA
AGATTCGGTGAGATGGAAGTTTGGGCATTACAAGCATATGGTGCTGCTTATACATTAAGA
GAGATGCTAACAGTTAAGTCAGATGACATAGCAGGTAGATCTAAAATGTATAAGAATATA
GTTGATGGTAAGTTGACTATGAATGTAGATGTTCCAGAATCATTCAATGTTTTAAGAAAT
GAAGTTAGAGCATTGGGTATAGATATGGATTTTGACTATTCATCTGAGGAAGAATAG
Protein sequence predicted based on nucleotide sequence
MSYSYAEKKRIRKEFGVLPHILDVPYLLSIQTESYKKFLTADAAKGRLHSGLEIVLKQSF PVESKNGQYELHYVDYQIGEPTFDETECQVRGATYDAPLNVKLRLVVYNKDALPNEKIVE DIREEYVYMGDIPLMTTNGTFIINGTERVVVSQLHRSPGVFFSKDDSEEGAFSARIIPYR GSWLDFEFDSKGIIWARIDRKRKFCATVILKALGYTQEQILENFSESKTITFNSKGFALR LDNLSNMKGELLKFDIVDAQDNVIVKKNKKLTSRDVKKIKDAGVDSVAIDFDLVSTLRVA KDIVNEATGEVIAYANDDVTESLLESCVEVGMLELEVIDFITTERGRYISDTLKYDLTRN TDEALVEIYKVLRPGDPPAAASVKALFEGLFFIESRYSLSDIGRMKLNARLGSDKVSKDI YTLENSDIVGVIEELINIRDGKGKVDDIDHLGNRRVRSVGEMVENQFRIGLYRVEKGIRE SMSLVHKDKLMPKDIVNSKPITAAIKEFFTSGALSQFMDQDNPLSEVTHKRRISALGPGG LSRDRAGFEVRDVHATHYGRLCPIETPEGPNIGLINSLASYARVNDYGFLEAPYRKVVDG KVTDEIEYLSAIDEDNYVIAQASTKLDENNHFVEDLIQCRSGGEAIFTESSRVQYMDVSA KQMVSAAAALIPFLEHDDANRVLMGANMQRQAVPTLKSEKPLVGTGMEKIVARDSGNCII ARNAGEVAEVDSNRIVIKVDTEKSQTSNLVDIYSLTKFKRSNKNTCINQRPIVNVGDKVE AGDILADGFATDFGELSLGHNLMVAFMPWNGYNFEDSILLSERIVKDDKYTSIHIEEFTC VARDTKLGPEEITADIPNVSESSLAKLDESGIVHIGANVEAGDILVAKITPKAEQQLTPE ERLLRAIFNEKASNVADSSLRMPSGTSGTVINVQVFENDKGGKSKRALKIEKELIDKARK DFDEEFAVIESVVKSSIEQEVVGAKIQKAKGLKKGAILTKEFLATLPFSKWLEISFEDEK LEEKVQNAREYYEEAKIAIDAKFEAKKKSITQSNELSPGVLKTVKVFVAIKKRIQPGDKM AGRHGNKGVVSRVLPVEDMPYMEDGTPVDVCLNPLGIPSRMNIGQILEAHLGLASYGLGK KIEKTLEKTRKAAELRKTLEEVYNSVGDKKVNLEALNDEEILTLCDNLKGGVPIATPVFD GAKEEDIKSLLKIGGFATNGQMKLFDGRTGKPFDRHVTVGYMYMLKLDHLVDDKMHARST GSYSLVTQQPLGGKAQFGGQRFGEMEVWALQAYGAAYTLREMLTVKSDDIAGRSKMYKNI VDGKLTMNVDVPESFNVLRNEVRALGIDMDFDYSSEEE*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4077
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDEST17
Synonyms:
pDEST-17
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and ribosome binding site (RBS), adds N-term 6xHis tag; amp resistance; recombinational cloning.
Comments:
None
Size (bp):
6354
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, in vitro transcription, recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
ATG
initiation ATG (before 6xHis tag)
101
103
bacterial origin
pBR322 ori
pBR322 origin of replication
3575
4248
gene fragment
ROP
ROP ORF
4619
4810
negative selection marker
ccdB
ccdB death cassette ONLY in Non-Recombined (NO INSERT) form
1374
1679
promoter
T7
T7 promoter
21
40
promoter
bla
bla promoter (prokaryotic promoter from bla gene)
2471
2569
recombination site
attR1
attR1 recombination site
140
264
recombination site
attR2
attR2 recombination site
1720
1844
ribosome binding site
RBS
Ribosome binding site
86
92
selectable marker
CmR
chlorampenicol resistance gene ONLY In Non-Recombined (NO INSERT) form
373
1032
selectable marker
AmpR
ampicillin resistance gene (bla)
2570
3430
tag
His
6xHis tag (N-terminal to insert)
113
130
trxn termination sequence
T7 term
T7 transcription termination sequence
1855
1983
