Gene:
Gene Symbol:
CHMP1B
Gene Name:
charged multivesicular body protein 1B
Original Clone ID:
WISP 14-08
Keyword:
"Mutation:a319g; g321t; g463c; a464g, Mutation:K107D D155R, CHMP1B K107D D155R protein (1-196) Sundquist lab internal ID corresponding to this construct = WISP 14-08, CHMP1B K107D D155R"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEX2T(TEV)
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
John McCullough
Wesley I. Sundquist
John McCullough
HsCD00671560
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 249nts
Open reading frame: 1 to 249
ATGGAGAAACACCTGTTCAACCTGAAGTTCGCGGCCAAAGAACTGAGTAGGAGTGCCAAA
AAATGCGATAAGGAGGAAAAGGCCGAAAAGGCCAAAATTGAAAAGGCCATTCAGAAGGGC
AACATGGAAGTTGCGAGGATACACGCCGAAAATGCCATCCGCCAGAAGAACCAGGCGGTG
AATTTCTTGAGAATGAGTGCGCGAGTCGATGCAGTGGCTGCCAGGGTCCAGACGGCGGTG
ACGATGGGCA
Protein sequence predicted based on nucleotide sequence
MEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIEKAIQKGNMEVARIHAENAIRQKNQAV NFLRMSARVDAVAARVQTAVTMG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEX2T(TEV)
Synonyms:
''
Description:
Bacterial Expression vector with an N-terminal GST tag and TEV site; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 01-69; Nde1, BamH1 restriction enzyme cloning can be used easily with this vector
Size (bp):
4993
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
primer
forward primer
pGEX forward sequencing primer GGTTCCGCGTGGATCTCCGG
920
939
primer
reverse primer
pGEX reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG
1043
1065
promoter
tac promoter
tac promoter
180
209
protease cleavage site
TEV
N-terminal TEV protease cleavage site
0
0
reporter gene
LacZ
LacZ alpha
4655
0
repressor binding site
lac operator
lac operator binding site
215
238
selectable marker
AmpR
Ampicillin resistance
1599
2259
tag
GST
N-terminal GST tag
258
929
