Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744763
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4278nts
Open reading frame: 1 to 4278
ATGAACGACTGGCACAGGATCTTCACCCAAAACGTGCTTGTCCCTCCCCACCCACAGAGA
GCGCGCCAGCCTTGGAAGGAATCCACGGCATTCCAGTGTGTCCTCAAGTGGCTGGACGGA
CCGGTAATTAGGCAGGGCGTGCTGGAGGTACTGTCAGAGGTTGAATGCCATCTGCGAGTG
TCTTTCTTTGATGTCACCTACCGGCACTTCTTTGGGAGGACGTGGAAAACCACAGTGAAG
CCGACGAAGCGACCGCCGTCCAGGATCGTCTTTAATGAGCCCTTGTATTTTCACACATCC
CTAAACCACCCTCATATCGTGGCTGTGGTGGAAGTGGTCGCTGAGGGCAAGAAACGGGAT
GGGAGCCTCCAGACATTATCCTGTGGGTTTGGAATTCTTCGGATCTTCAGCAACCAGCCG
GACTCTCCTATCTCTGCTTCCCAGGACAAAAGGTTGCGGCTGTACCATGGCACCCCCAGA
GCCCTCCTGCACCCGCTTCTCCAGGACCCCGCAGAGCAAAACAGACACATGACCCTCATT
GAGAACTGCAGCCTGCAGTACACGCTGAAGCCACACCCGGCCCTGGAGCCTGCGTTCCAC
CTTCTTCCTGAGAACCTTCTGGTGTCTGGTCTGCAGCAGATACCTGGCCTGCTTCCAGCT
CATGGAGAATCCGGCGACGCTCTCCGAAAGCCTCGCCTCCAGAAGCCCATCACGGGGCAC
TTGGATGACTTATTCTTCACCCTGTACCCCTCCCTGGAGAAGTTTGAGGAAGAGCTGCTG
GAGCTCCACGTCCAGGACCACTTCCAGGAGGGATGTGGCCCACTGGACGGTGGTGCCCTG
GAGATCCTGGAGCGGCGCCTGCGTGTGGGCGTGCACAATGGTCTGGGCTTCGTGCAGCGG
CCGCAGGTCGTTGTACTGGTGCCTGAGATGGATGTGGCCTTGACGCGCTCAGCTAGCTTC
AGCAGGAAAGTGGTTTCCTCTTCCAAGACCAGCTCCGGGAGCCAAGCTCTGGTTTTGAGA
AGCCGCCTCCGCCTCCCAGAGATGGTCGGCCACCCTGCATTTGCGGTCATCTTCCAGCTG
GAGTACGTGTTCAGCAGCCCTGCAGGAGTGGACGGCAATGCAGCTTCGGTCACCTCTCTG
TCCAACCTGGCATGCATGCACATGGTCCGCTGGGCTGTTTGGAACCCCTTGCTGGAAGCT
GATTCTGGAAGGGTGACCCTGCCTCTGCAGGGTGGGATCCAGCCCAACCCCTCGCACTGT
CTGGTCTACAAGGTGCCCTCAGCCAGCATGAGCTCTGAAGAGGTGAAGCAAGTGGAGTCG
GGTACACTGCGGTTCCAGTTCTCGCTGGGCTCAGAAGAACACCTGGATGCACCCACGGAG
CCTGTCAGTGGCCCCAAAGTGGAGCGGCGGCCTTCCAGGAAACCACCCACGTCCCCTTCG
AGCCCGCCAGCGCCAGTACCTCGAGTTCTCGCTGCCCCGCAGAACTCACCTGTGGGACCA
GGGTTGTCAATTTCCCAGCTGGCGGCCTCCCCGCGGTCCCCGACTCAGCACTGCTTGGCC
AGGCCTACTTCACAGCTACCCCATGGCTCTCAGGCCTCCCCGGCCCAGGCACAGGAGTTC
CCGTTGGAGGCCGGTATCTCCCACCTGGAAGCCGACCTGAGCCAGACCTCCCTGGTCCTG
GAAACATCCATTGCCGAACAGTTACAGGAGCTGCCGTTCACGCCTTTGCATGCCCCTATT
GTTGTGGGAACCCAGACCAGGAGCTCTGCAGGGCAGCCCTCGAGAGCCTCCATGGTGCTC
CTGCAGTCCTCCGGCTTTCCCGAGATTCTGGATGCCAATAAACAGCCAGCCGAGGCTGTC
AGCGCTACAGAACCTGTGACGTTTAACCCTCAGAAGGAAGAATCAGATTGTCTACAAAGC
AACGAGATGGTGCTACAGTTTCTTGCCTTTAGCAGAGTGGCCCAGGACTGCCGAGGAACA
TCATGGCCAAAGACTGTGTATTTCACCTTCCAGTTCTACCGCTTCCCACCCGCAACGACG
CCACGACTGCAGCTGGTCCAGCTGGATGAGGCCGGCCAGCCCAGCTCTGGCGCCCTGACC
CACATCCTCGTGCCTGTGAGCAGAGATGGCACCTTTGATGCTGGGTCACCTGGCTTCCAG
CTGAGGTACATGGTGGGCCCTGGGTTCCTGAAGCCAGGTGAGCGACGCTGCTTTGCCCGC
TACCTGGCCGTGCAGACCCTGCAGATTGACGTCTGGGACGGAGACTCCCTGCTGCTCATC
GGATCTGCTGCCGTCCAGATGAAGCATCTCCTCCGCCAAGGCCGGCCGGCTGTGCAGGCC
TCCCACGAGCTTGAGGTCGTGGCAACTGAATACGAGCAGGACAACATGGTGGTGAGTGGA
GACATGCTGGGGTTTGGCCGCGTCAAGCCCATCGGCGTCCACTCGGTGGTGAAGGGCCGG
CTGCACCTAACTTTGGCCAACGTGGGTCACCCGTGTGAACAGAAAGTGAGAGGTTGTAGC
ACATTGCCACCGTCCAGATCTCGGGTCATCTCAAACGATGGAGCCAGCCGTTTCTCTGGA
GGCAGCCTCCTCACGACTGGAAGCTCAAGGCGAAAACACGTGGTGCAAGCACAGAAGCTG
GCGGACGTGGACAGTGAGCTGGCTGCCATGCTACTGACCCATGCCCGGCAGGGCAAGGGG
CCCCAGGACGTCAGCCGCGAGTCGGATGCCACCCGCAGGCGTAAGCTGGAGCGGATGAGG
TCTGTGCGCCTGCAGGAGGCCGGGGGAGACTTGGGCCGGCGCGGGACGAGCGTGTTGGCG
CAGCAGAGCGTCCGCACACAGCACTTGCGGGACCTACAGGTCATCGCCGCCTACCGGGAA
CGCACGAAGGCCGAGAGCATCGCCAGCCTGCTGAGCCTGGCCATCACCACGGAGCACACG
CTCCACGCCACGCTAGGGGTCGCCGAGTTCTTTGAGTTTGTGCTTAAGAACCCCCACAAC
ACACAGCACACGGTGACTGTGGAGATCGACAACCCCGAGCTCAGCGTCATCGTGGACAGT
CAGGAGTGGAGGGACTTCAAGGGTGCTGCTGGCCTGCACACACCGGTGGAGGAGGACATG
TTCCATCTGCGTGGCAGCCTGGCCCCCCAGCTCTACCTGCGCCCCCACGAGACTGCCCAC
GTCCCCTTCAAGTTCCAGAGCTTCTCTGCAGGGCAGCTGGCCATGGTGCAGGCCTCTCCT
GGGTTGAGCAACGAGAAGGGCATGGACGCCGTGTCACCTTGGAAGTCCAGCGCAGTGCCC
ACTAAACACGCCAAGGTCTTGTTCCGAGCGAGTGGTGGCAAGCCCATCGCCGTGCTCTGC
CTGACTGTGGAGCTGCAGCCCCACGTGGTGGACCAGGTCTTCCGCTTCTATCACCCGGAG
CTCTCCTTCCTGAAGAAGGCCATCCGCCTGCCGCCCTGGCACACATTTCCAGGTGCTCCG
GTGGGAATGCTTGGTGAGGACCCCCCAGTCCATGTTCGCTGCAGCGACCCGAACGTCATC
TGTGAGACGCAGAATGTGGGCCCCGGGGAACCACGGGACATATTTCTGAAGGTGGCCAGT
GGTCCAAGCCCGGAGATCAAAGACTTCTTTGTCATCATTTACTCGGATCGCTGGCTGGCG
ACACCCACACAGACGTGGCAGGTCTACCTCCACTCCCTGCAGCGCGTGGATGTCTCCTGC
GTCGCAGGCCAGCTGACCCGCCTGTCCCTTGTCCTTCGGGGGACACAGACAGTGAGGAAA
GTGAGAGCTTTCACCTCTCATCCCCAGGAGCTGAAGACAGACCCCAAAGGTGTCTTCGTG
CTGCCGCCTCGTGGGGTGCAGGACCTGCATGTTGGCGTGAGGCCCCTTAGGGCCGGCAGC
CGCTTTGTCCATCTCAACCTGGTGGACGTGGATTGCCACCAGCTGGTGGCCTCCTGGCTC
GTGTGCCTCTGCTGCCGCCAGCCGCTCATCTCCAAGGCCTTTGAGATCATGTTGGCTGCG
GGCGAAGGGAAGGGTGTCAACAAGAGGATCACCTACACCAACCCCTACCCCTCCCGGAGG
ACATTCCACCTACACAGCGACCACCCGGAGCTGCTGCGGTTCAGAGAGGACTCCTTCCAG
GTCGGGGGTGGAGAGACATACACCATCGGCTTGCAGTTTGCGCCTAGTCAGAGAGTGGGT
GAGGAGGAGATCCTGATCTACATCAATGACCATGAGGACAAAAACGAAGAGGCATTTTGC
GTGAAGGTCATCTACCAG
Protein sequence predicted based on nucleotide sequence
MNDWHRIFTQNVLVPPHPQRARQPWKESTAFQCVLKWLDGPVIRQGVLEVLSEVECHLRV SFFDVTYRHFFGRTWKTTVKPTKRPPSRIVFNEPLYFHTSLNHPHIVAVVEVVAEGKKRD GSLQTLSCGFGILRIFSNQPDSPISASQDKRLRLYHGTPRALLHPLLQDPAEQNRHMTLI ENCSLQYTLKPHPALEPAFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPITGH LDDLFFTLYPSLEKFEEELLELHVQDHFQEGCGPLDGGALEILERRLRVGVHNGLGFVQR PQVVVLVPEMDVALTRSASFSRKVVSSSKTSSGSQALVLRSRLRLPEMVGHPAFAVIFQL EYVFSSPAGVDGNAASVTSLSNLACMHMVRWAVWNPLLEADSGRVTLPLQGGIQPNPSHC LVYKVPSASMSSEEVKQVESGTLRFQFSLGSEEHLDAPTEPVSGPKVERRPSRKPPTSPS SPPAPVPRVLAAPQNSPVGPGLSISQLAASPRSPTQHCLARPTSQLPHGSQASPAQAQEF PLEAGISHLEADLSQTSLVLETSIAEQLQELPFTPLHAPIVVGTQTRSSAGQPSRASMVL LQSSGFPEILDANKQPAEAVSATEPVTFNPQKEESDCLQSNEMVLQFLAFSRVAQDCRGT SWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGFQ LRYMVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQA SHELEVVATEYEQDNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCS TLPPSRSRVISNDGASRFSGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKG PQDVSRESDATRRRKLERMRSVRLQEAGGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRE RTKAESIASLLSLAITTEHTLHATLGVAEFFEFVLKNPHNTQHTVTVEIDNPELSVIVDS QEWRDFKGAAGLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFKFQSFSAGQLAMVQASP GLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQVFRFYHPE LSFLKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVAS GPSPEIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRK VRAFTSHPQELKTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWL VCLCCRQPLISKAFEIMLAAGEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQ VGGGETYTIGLQFAPSQRVGEEEILIYINDHEDKNEEAFCVKVIYQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
