Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments:
None
Publications:
PMID:16377204 Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics