Detailed Vector Information: pCMV4
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with CMV promoter, AMV4-A translational enhancer; ampicillin resistance; restriction enzyme cloning.
Properties:
mammalian expression, multiple cloning site
Author Name:
S. Andersson
Publications:
Title: Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme
Type
Name
Description
Start
End
MCS
MCS
MCS (BglII, KpmI, MluI, ClaI, HindIII, XbaI, SmaI)
0
0
bacterial origin
unknown ori
unknown bacterial origin
0
0
phage origin
F1 ori
F1 phage origin for ssDNA production
0
0
poly-A signal
hGH polyA
human growth hormone polyadenylation signal sequence
0
0
promoter
CMV
human cytomegalovirus (CMV) promoter
0
0
selectable marker
AmpR
ampicillin resistance gene from E. coli
0
0
trln enhancer
AMV4-A
Alfalfa mosaic virus 4 RNA translational enhancer (A)
0
0
trxn termination sequence
hGH trxn term
human growth hormone gene transcription termination sequence
0
0
viral origin
SV40 ori
SV40 origin of DNA replication and early region enhancer sequences
0
0