Vector Name:
pSP72 syn-CTAP AphAI
Synonyms:
pSP72-PTP-AphAI, pSP72-PTP-KanR
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type form); kanamycin resistance; restriction enzyme cloning.
Comments:
See pSP72-syn-CTAP for map without the kanR (AphAI) insert and I-SceI site that make this useful for BAC mutagenesis
Size (bp):
3968
Parent Vector:
None
Properties:
BAC mutagenesis, I-SceI site, Red recombination, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
I-SceI site
I-SceI endonuclease cleavage site
263
280
MCS
I-SceI site
I-SceI endonuclease cleavage site
263
280
MCS
MCS
MCS (XbaI, BamHI, KpnI, EcoRI, BglII)
1547
1591
bacterial origin
ColE1 ori
ColE1-type origin of replication
1864
2546
binding site
IgG 1
IgG bind site
61
234
binding site
IgG 2
IgG bind site
1273
1458
primer site
T7
T7 primer bind site/promoter
1605
1624
recombination site
recomb site 1
50 bp recombination substrate (Red strategy)
213
262
recombination site
recomb site 2
50 bp recombination substrate (Red strategy)
1251
1300
selectable marker
AmpR
ampicillin resistance gene
2644
3303
selectable marker
KanR
AphAI kanamycin resistance gene
263
1250
tag
Protein C
Protein C fragment (affinity tag fragment)
1510
1545
