Vector Name:
pDB.His.TRX
Synonyms:
pHX
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector that adds N-terminal 6xHis tag, thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5720
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Berkeley Structural Genomics Center
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1421
2103
promoter
T7
T7 promoter
4984
5003
protease cleavage site
TEV
TEV protease cleavage site
5458
5475
repressor binding site
lac operator
lacO sequence region
5003
5025
repressor protein gene
lacI
lacI coding region
3518
4597
restriction site
CpoI
CpoI restriction site
5202
5208
selectable marker
KanR
kanamycin resistance gene
563
1375
ssDNA origin
f1 ori
f1 origin
29
335
tag
His
6xHis tag
5083
5100
tag
TRX
thioredoxin tag
5107
5430
trxn termination sequence
T7 term
T7 terminator sequence
5649
5695
