Vector Name:
pDB.TRX
Synonyms:
pTRX, pX
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5693
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Berkeley Structural Genomics Center
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
f1
F1 origin
29
335
bacterial origin
ColE
ColE1 origin
1421
2103
promoter
T7
T7 promoter
4984
5003
protease cleavage site
TEV
TEV protease cleavage site cds
5431
5448
repressor binding site
lac operator
lac operator
5003
5025
repressor protein gene
LacI
LacI gene
2515
4594
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
TRX
thioredoxin cds tag
5080
5403
trxn termination sequence
T7 term
T7 term sequencing primer
5610
5628
