Vector Name:
pEU-GST-FV
Synonyms:
''
Sequencing Primer:
Forward:
GST Forward
Reverse:
pEU Reverse
Description:
Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
5612
Parent Vector:
None
Properties:
cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3081
3763
gene fragment
Omega
Omega
25
148
gene fragment
tet operator
TMV-L
2466
2526
gene fragment
Barnase
BarCat cassette, including Barnase
863
1199
gene fragment
CAT
BarCat cassette, including CAT
1301
1958
promoter
SP6
SP6 promoter
1
18
protease cleavage site
3C
3C cleavage site cds
812
835
recombination site
MCS
pF1K (Flexi-Vector) homology domain
1966
2094
selectable marker
AmpR
ampicillin resistance gene
3861
4520
tag
GST
GST coding sequence (GST cds)
149
805
trxn termination sequence
T7 term
T7 terminator sequence
2075
2094
