Detailed Vector Information: pMH1
| Vector Name: | pMH1 | Powered by LabGenius |
|---|---|---|
| Synonyms: | pMH-1 |
pMH1 |
| Description: | Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning. | |
| Comments: | None | |
| Size (bp): | 4048 | |
| Empty Vector: | None | |
| Parent Vector: | None | |
| Properties: | PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker | |
| Author Name: |
JCSG |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
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| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | ori | ColE1-type bacterial origin of replication | 1887 | 2569 |
| inducible trxn activator protein gene | araC | araC cds | 3144 | 4019 |
| promoter | araC | araC promoter | 256 | 285 |
| promoter | pBAD | pBAD promoter | 250 | 277 |
| selectable marker | AmpR | ampicillin resistance gene | 1130 | 1789 |
| tag | His | N-terminal 6xHis tag | 364 | 381 |
| trxn regulatory element | arabinose O1 operator | arabinose O1 operator | 161 | 182 |
| trxn regulatory element | arabinose O2 operator | arabinose O2 operator | 5 | 23 |
| trxn termination sequence | rrnB | rrnB T2 transcription terminator | 794 | 820 |