Detailed Vector Information: pVP33A

Vector Name:
pVP33A
Synonyms:
''
Sequencing Primer:
Forward:  MBP Forward
Reverse:  T7 Terminator
Description:
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
7228
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ori
pBR322-type bacterial origin of replication
5422
6104
gene fragment
BarCat-bar
Barnase cds
1381
1717
gene fragment
BarCat-cat
CAT cds - chloramphenicol resistance
1819
2476
primer site
T7 rev
T7 reverse primer site
2533
2551
promoter
T5
T5 promoter
43
89
promoter
T5 pr 2
T5 promoter (two of two)
0
0
protease cleavage site
3C
3CP protease cleavage site cds
1293
1317
protease cleavage site
TEV
TEV cleavage site cds
1335
1355
recombination site
pF1K site
pF1K homology site
2484
2612
repressor protein gene
LacIq
LacIq
3762
4841
selectable marker
AmpR
ampicillin resistance gene
6202
6861
tag
His
8xHis epitope tag
157
180
tag
MBP
MBP fusion protein tag
187
1283
tag
tetraCys
tetraCyc cds
1318
1335