Vector Name:
pH3c-LIC
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Size (bp):
5382
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
Marta Pioletti
Center for Structures of Membrane Proteins
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
5162
5167
phage origin
f1
f1 phage origin
29
335
promoter
T7
T7 promoter
4984
5003
protease cleavage site
3C
3C protease cleavage site; separates the His tag from the insert
5132
5155
selectable marker
KanR
Kanamycin resistance gene
563
1066
tag
His
N-terminal 8xHis tag
5075
5098
trxn termination sequence
T7 term
T7 transcription termination sequence
5316
5362
