Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning.
Comments:
Requires growth in ccdB survival strain
Size (bp):
5936
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
PSI
New York Consortium on Membrane Protein Structure