Vector Name:
pMA507
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
E. coli expression vector with a T7 promoter, N-terminal 6xHis tag and TEV protease cleavage site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
Comments:
Requires special host strain E. coli DB3.1 for growth
Size (bp):
6046
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
David Eisenberg
Mark Arbing
Integrated Center for Structure and Function Innovation
PSI
Mark Arbing
Integrated Center for Structure and Function Innovation
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial operon
lac operator
Lac operator CDS
5003
5027
bacterial origin
ColE
ColE1 pBR322 origin
2084
2084
death cassette
ccdB
ccdB death cassette
5134
5883
phage origin
f1
f1 phage origin
12
467
primer
T7 forward
T7 forward sequencing primer
4985
5001
primer
T7 term primer
T7 terminator reverse primer
5963
5981
promoter
T7
T7 promoter
4984
5000
protease cleavage site
TEV
TEV protease cleavage site
5113
5133
repressor protein gene
LacI
lacI regulatory gene
3518
4597
ribosome binding site
RBS
ribosomal binding site
5055
5063
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
N terminal 6xHis tag
5095
5112
trxn termination sequence
T7 term
T7 terminator
5975
6021
