Detailed Vector Information: pMYC5-H

Vector Name:
pMYC5-H
Synonyms:
''
Sequencing Primer:
Forward:  Phsp
Reverse:  Unknown
Description:
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI).
Comments:
Target gene controlled by Phsp promoter for expression is cloned between NdeI and SbfI restriction sites. Modified from PMYC4H with unique SrfI site.
Size (bp):
5157
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
457
563
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
1242
1776
mycobacterial origin
myc ori
mycobacterial origin of replication
1963
3808
promoter
Phsp
Phsp promoter
77
456
selectable marker
HygR
hygromycin resistance cassette
4145
5143
tag
His
C-terminal His tag
500
532
tag
myc
C-terminal c-myc tag
500
535
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
42
62
trxn termination sequence
T4g32
T4g32 transcription terminator
578
598