Vector Name:
pMCSG32
Synonyms:
''
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and C-terminal 6xHis, TEV-protease cleavage site, N-terminal MBP tag with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments:
None
Size (bp):
6418
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Publications:
PMID:
20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
4412
4412
primer
T7 forward
T7 forward sequencing primer
1491
1507
primer
T7 reverse
T7 terminator reverse sequencing primer
66
83
promoter
T7
T7 promoter
1492
1508
protease cleavage site
TEV
TEV protease cleavage site
232
252
protease cleavage site
TVMV
TVMV cleavage site
263
283
recombination site
LIC
LIC site
245
265
repressor binding site
lac operator
LacO fragment
1467
1489
repressor protein gene
LacI
Lac I cds
2022
2981
selectable marker
AmpR
ampicillin resistance marker
5170
6030
tag
MBP
N-terminal maltose binding protein (MBP) tag
314
1420
tag
His 2
His Tag;carboxy terminal His tag from pET-21a
140
157
tag
His
C-terminal 6xHis tag
208
225
trxn termination sequence
T7 term
T7 terminator
26
72
