Vector Name:
pJFT7_nHalo_empty
Synonyms:
''
Sequencing Primer:
Forward:
NAP150
Reverse:
T7 terminator
Description:
In vitro expression vector with T7 promoter and N-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning
Comments:
Modified from pANT7_cGST, expresses Halo without an insert
Size (bp):
6166
Parent Vector:
None
Properties:
cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Fernanda Festa
Center for Personalized Diagnostics
Justin Saul
Fernanda Festa
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
1766
2448
bacterial origin
M13 Ori
M13 origin
3842
4297
enhancer
CITE
cap-independent translational enhancer (CITE)
17
515
primer
HaloseqF
HaloseqF forward sequencing primer aagcctgcctaactgcaa
1289
1306
primer
M13 forward
M13 forward sequencing primer
4441
4458
primer
NAP150
NAP150 forward sequencing primer cccattgtatgggatctgatc
388
408
promoter
T7
T7 promoter
4457
4481
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
1422
1442
selectable marker
LacZ
LacZ alpha gene
4302
4370
selectable marker
AmpR
Ampicillin resistance gene
2546
3205
tag
HALO
N-terminal Halo Tag
519
1409
trxn termination sequence
T7 term
T7 terminator
1526
1544
