Vector Name:
pJFT7_cHalo_DC
Synonyms:
''
Description:
In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
Modified from pANT7_cGST, can be used for either Gateway or Flexi cloning, contains Gateway Death Cassette
Size (bp):
6205
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Center for Personalized Diagnostics
Joshua LaBaer
Justin Saul
Fernanda Festa
Joshua LaBaer
Justin Saul
Fernanda Festa
Publications:
PMID:
201200062
Title: A versatile protein microarray platform enabling antibody profiling against denatured proteins.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3490
4172
bacterial origin
M13 Ori
M13 origin
5566
3021
death cassette
ccdB
ccdB death cassette (removed in clones with insert)
556
2210
death cassette
ccdB (BsrGI removed)
ccdB death cassette with BsrGI restriction site removed
1765
2070
enhancer
CITE
cap-independent translational enhancer (CITE)
17
515
primer
M13 forward
M13 forward sequencing primer
6165
6182
primer
NAP150
NAP150 forward sequencing primer cccattgtatgggatctgatc
388
408
primer
cHaloseqR
cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT
2351
2369
promoter
T7
T7 promoter
6181
6205
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
2258
2278
recombination site
attR1
recombinational cloning site
531
555
recombination site
attR2
recombinational cloning site
2211
2235
reporter gene
LacZ
LacZ alpha gene
6026
6094
selectable marker
AmpR
ampicillin resistance gene
4270
4929
selectable marker
CmR
chloramphenicol resistance gene (removed in plasmids with insert)
764
1420
tag
HALO
C-terminal Halo tag
2294
3178
trxn termination sequence
T7 term
T7 termination
3250
3268
