Detailed Vector Information: pMCSG75

Vector Name:
pMCSG75
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region of pMCSG62 replaced by LIC region of pMCSG28 with the addition of the biotinylation site. Product is a C-terminal biotinylation vector.
Size (bp):
5876
Parent Vector:
Name: pMCSG62
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3851
4533
gene fragment
BirA
This protein activates biotin and transfers the biotin moiety to biotin-accepting proteins
241
1206
ligation independent cloning
LIC
ligation independent cloning site
1346
1366
promoter
T7
T7 promoter
1428
1444
protease cleavage site
TEV
TEV protease cleavage site
1333
1353
repressor protein gene
LacI
LacI
2481
3440
ribosome binding site
RBS
ribosome binding site
1215
1220
selectable marker
AmpR
ampicillin resistance
4628
5488
signal sequence
biotinylation
biotinylation signal
1282
1326
tRNA
ileX
ileX rare tRNA
2027
2178
tRNA
argU
argU rare tRNA
1511
1867
tag
His
6xHis tag
1258
1275
trxn termination sequence
T7 term
T7 terminator
26
72