Detailed Vector Information: pRSET-natGFPHis

Vector Name:
pRSET-natGFPHis
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  GFPR
Description:
Bacterial expression vector with a T7 promoter; adds C-terminal GFP-His6 tag; ampicillin resistance in bacteria; ligation independent cloning
Comments:
Based on Invitrogen pRSET B. Vector for subcloning to produce C-terminal GSAGSAAGSGEF linker + GFP tag. Insert not expressed due to ~0.5 kb BseRI-flanked sequence, containing stop codons in all three frames, between the RBS and GFP. Subclone using BseRI digestion and Clontech In-Fusion ligase-independent cloning. See Martin-Garcia et al 2014 Biochemistry 53:1958, PMID 24593131
Size (bp):
3998
Parent Vector:
None
Properties:
bacterial expression, fluorescent marker, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
PMID: 24593131
Title: Purification and Biophysical Characterization of the CapA Membrane Protein FTT0807 from Francisella tularensis.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin
3148
3821
primer
reverse primer
GFPR reverse sequencing primer TCTCCACTGACAGAAAATTTGTGC
642
665
promoter
T7
T7 promoter
20
39
ribosome binding site
RBS
ribosomal binding site
85
92
selectable marker
AmpR
ampicillin resistance
2143
3003
ssDNA origin
f1
f1 origin
1557
2012
tag
GFP
C-terminal GFP tag
568
1284
tag
His
C-terminal 6xHis tag
1585
1302
trxn termination sequence
T7 term
T7 terminator
1357
1486