Bacterial expression vector with a T7 promoter; adds C-terminal GFP-His6 tag; ampicillin resistance in bacteria; ligation independent cloning
Comments:
Based on Invitrogen pRSET B. Vector for subcloning to produce C-terminal GSAGSAAGSGEF linker + GFP tag. Insert not expressed due to ~0.5 kb BseRI-flanked sequence, containing stop codons in all three frames, between the RBS and GFP. Subclone using BseRI digestion and Clontech In-Fusion ligase-independent cloning. See Martin-Garcia et al 2014 Biochemistry 53:1958, PMID 24593131
Size (bp):
3998
Parent Vector:
None
Properties:
bacterial expression, fluorescent marker, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Center for Membrane Proteins in Infectious Diseases
PSI:Biology