Vector Name:
pFastBac1-OSFP
Synonyms:
WISP13-86
Sequencing Primer:
Forward:
FasBacFwd
Reverse:
FastBacRev
Description:
Baculoviral entry vector with polyhedron promoter for gateway recombination in DHB10Bac cells to produce bacmid; ampicillin resistance in bacterial; SLIC cloning into SmaI site
Comments:
Empty vector Sundquist Lab internal database number = WISP13-86
Size (bp):
4959
Parent Vector:
None
Properties:
baculovirus/insect cell expression, ligation independent cloning (LIC)
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
3pUTR
2 micron ori
FastBacRev reverse sequencing primer GATCCTCTAGTACTTCTCG
4328
4346
bacterial origin
ColE
ColE1 bacterial origin
1544
2226
poly-A signal
SV40 PolyA
SV40 late polyA
4388
4579
primer
forward primer
FasBacFwd forward sequencing primer ATACCGTCCCACCATCG
4010
4026
promoter
PH
Polyhedrin promoter
3904
4032
selectable marker
AmpR
Ampicillin resistance
787
1446
selectable marker
GenR
Gentamicin resistance
3000
3533
ssDNA origin
f1
f1 origin
19
325
transposon fragment
Tn7L
Tn7L
2709
2933
