Vector Name:
pCDF-BAD
Synonyms:
''
Sequencing Primer:
Forward:
ARAC5R
Reverse:
T7 terminator
Description:
Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI
Comments:
For arabinose-inducible expression of the insert, on a vector that is compatible with most other cloning vectors (non-ColE1 origin of replication; Clone by ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI
Size (bp):
3552
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Arizona State University
Medical University of South Carolina
Debra T. Hansen
Jeffrey L. Hansen
Thirumagal Thiyagarajan
Medical University of South Carolina
Debra T. Hansen
Jeffrey L. Hansen
Thirumagal Thiyagarajan
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
CloDF13
CloDF13 origin
2792
3530
primer
forward primer
ARAC5R forward sequencing primer GGGATCATTTTGCGCTTCAG
694
683
promoter
araC promoter
araC promoter
1138
1166
promoter
arabinose BAD
arabinose BAD promoter
1263
1290
repressor binding site
arabinose O2 operator
arabinose O2 operator
1016
1033
repressor protein gene
araC
araC
109
987
ribosome binding site
RBS
ribosome binding site
2658
2662
selectable marker
SpectR
Spectinomycin, streptomycin resistance
2717
2722
trxn termination sequence
T7 term
T7 terminator
1643
1690
