Detailed Vector Information: pMK-Dest

Vector Name:
pMK-Dest
Synonyms:
''
Sequencing Primer:
Forward:  CMV forward
Reverse:  None
Description:
with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
Comments:
From Karen Anderson Lab, Produced by Bianca Varda, Krishna, and Mark Knappenberger
Size (bp):
3386
Parent Vector:
Name: pMax-Cloning
Description:
Properties:
Gateway, bacterial expression, with tag/fusion/marker
Author Name:
Center for Personalized Diagnostics
Bianca
Mark Knappenberger
Krishna
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available

Vector Features:

Type
Name
Description
Start
End
CDS
TEV
TEV Cleavage site
1252
1272
Origin
pBR322 origin
2748
2130
primer
CMV-F
949
969
promoter
CMV
938
1019
promoter
lac promoter
1437
1460
recombination site
attR1
1288
1412
recombination site
attR2
1412
1312
recombination site
AttB2
2066
2090
selectable marker
NeoR
Neomycin/Kanamycin Resistance
2816
209