Vector Name:
pAH_DEST_nHalo-nTEV-3xFlag
Synonyms:
''
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination) clone, mammalian expression expression with CMV enhancer and chicken_beta_actin_promoter-var002 and chimeric intron and HaloTag(R) and TEV and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and 3xFlag and 8xHis and beta globin and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
7403
Parent Vector:
None
Properties:
Gateway, acceptor (destination), mammalian expression, with tag/fusion/marker
Author Name:
Vel Murugan
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
HaloTag(R)
modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
1816
2706
CDS
TEV
tobacco etch virus (TEV) protease recognition and cleavage site
2737
2757
CDS
CmR
confers resistance to chloramphenicol
2991
3650
CDS
ccdB
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
3992
4297
CDS
3xFlag
three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
4464
4529
CDS
8xHis
8xHis affinity tag
4641
4664
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
6412
7272
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
4
383
intron
chimeric intron
chimera between introns from chicken beta-actin and rabbit beta-globin
662
1674
poly-A signal
beta globin
rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)
4825
4880
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
5239
5255
promoter
chicken_beta_actin_promoter-var002
NULL
386
661
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2907
2937
promoter
lac promoter
promoter for the E. coli lac operon
5287
5317
promoter
AmpR promoter
NULL
7273
7377
protein binding site
attR1
recombination site for the Gateway® LR reaction
2758
2882
protein binding site
attR2
recombination site for the Gateway® LR reaction
4338
4462
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
5263
5279
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
5332
5353
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
5653
6241
