Vector Name:
pAH_nHalo_3xFlag
Synonyms:
''
Description:
with tag/fusion/marker assay, restriction enzyme clone, mammalian expression expression with CMV enhancer and chicken_beta_actin_promoter-var002 and chimeric intron and HaloTag(R) and 3xFlag and beta globin and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
5541
Parent Vector:
None
Properties:
mammalian expression, restriction enzyme, with tag/fusion/marker
Author Name:
Vel Murugan
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
HaloTag(R)
modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
1816
2706
CDS
3xFlag
three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
2737
2802
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
4550
5410
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
4
380
intron
chimeric intron
chimera between introns from chicken beta-actin and rabbit beta-globin
662
1674
poly-A signal
beta globin
rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)
2963
3018
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3377
3393
promoter
chicken_beta_actin_promoter-var002
NULL
386
661
promoter
lac promoter
promoter for the E. coli lac operon
3425
3455
promoter
AmpR promoter
NULL
5411
5515
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
3401
3417
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
3470
3491
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3791
4379
