Vector Name:
pAH_DEST_nHalo-cTEV-8xHis
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination) clone, mammalian expression expression with CMV enhancer and chicken_beta_actin_promoter-var002 and Chimeric Guide RNA Scaffold and HALO and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and TEV and 8xHis and -10 AP and beta globin and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
7364
Parent Vector:
None
Properties:
Gateway, acceptor (destination), mammalian expression, with tag/fusion/marker
Author Name:
Vel Murugan
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
HALO
modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
1816
2706
CDS
CmR
confers resistance to chloramphenicol
2970
3629
CDS
ccdB
product is CcdB, a bacterial toxin that poisons DNA gyrase
3971
4276
CDS
TEV
tobacco etch virus (TEV) protease recognition and cleavage site
4443
4463
CDS
8xHis
8xHis affinity tag
4467
4490
CDS
-10 AP
8xHis affinity tag
4602
4625
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
6373
7233
Replication Origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
5614
6202
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
4
383
intron
Chimeric Guide RNA Scaffold
chimera between introns from chicken beta-actin and rabbit beta-globin
662
1674
poly-A signal
beta globin
rabbit beta-globin polyadenylation signal
4786
4841
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
5200
5216
promoter
chicken_beta_actin_promoter-var002
promoter
386
661
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2886
2916
promoter
lac promoter
promoter for the E. coli lac operon
5248
5278
promoter
AmpR promoter
gene is bla
7234
7338
protein binding site
attR1
recombination site for the Gateway LR reaction
2737
2861
protein binding site
attR2
recombination site for the Gateway LR reaction
4317
4441
protein binding site
lac operator
binds lac repressor encoded by lacI
5224
5240
protein binding site
CAP binding site
binds E. coli catabolite activator protein
5293
5314
